70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

Enzymes (1)

EC N°
Additive Chemical formula, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

E 1600

3-phytase (EC 3.1.3.8)

Preparation of 3-phytase produced by Aspergillus niger (CBS 114.94) having a minimum phytase activity of:

Solid form: 5000 FTU ⁽¹⁾/g

Liquid form: 5000 FTU ⁽¹⁾/ml

Piglets

2 months

500 FTU⁽¹⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500 FTU⁽¹⁾.

3. For use in compound feed containind more than 0,23% phytin bound phosphorus

Without a time limit

Pigs for fattening

-

280 FTU⁽¹⁾

^-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 400-500 FTU⁽¹⁾.

3. For use in compound feed containind more than 0,23% phytin bound phosphorus

Without a time limit

Sows

-

500 FTU⁽¹⁾

^-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500 FTU⁽¹⁾.

3. For use in compound feed containind more than 0,36% phytin bound phosphorus

Without a time limit

Chickens for fattening

-

375 FTU⁽¹⁾

^-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500-700 FTU⁽¹⁾.

3. For use in compound feed containind more than 0,23% phytin bound phosphorus

Without a time limit

Laying hens

-

250 FTU⁽¹⁾

^-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 300-400 FTU⁽¹⁾.

3. For use in compound feed containind more than 0,23% phytin bound phosphorus

Without a time limit

(1) FTU is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute fom sodium phytate at pH 5,5 and 37°C.

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (1)

N°
Additive Chemical name, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

1

3-phytase (EC 3.1.3.8)

Preparation of 3-phytase produced by Aspergillus niger (CBS 114.94) having a minimum phytase activity of 5000 FTU ⁽¹⁾/g for solid and liquid preparations

Turkeys

-

125 FTU⁽¹⁾

-

1. Indicate in the directions for use for the additive and the premixture the storage temperature, the storage duration and stability on pelleting.

2. Recommended dose per kg of complete feedingstuff: 200 to 800 FTU⁽¹⁾.

3. For use in compound feedingstuffs with a minimum content of 0,3% phytase, e.g. 20% wheat.

14.12.2003 ^(I)(a)

2

3-phytase (EC 3.1.3.8)

Preparation of 3-phytase produced by Aspergillus oryzae (DMS 10 289) having a minimum activity of:

Coated form: 2500 FYT⁽²⁾/g

Liquid form: 5000 FYT⁽²⁾/g

Laying hens

-

500 FYT ⁽²⁾

1000 FYT⁽²⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 750 FYT ⁽²⁾.

3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.

30.6.2004 ^(I)(b)

Piglets

Four months

250 FYT⁽²⁾

1000 FYT⁽²⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500 FYT ⁽²⁾.

3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.

30.6.2004 ^(I)(c)

Pigs for fattening

-

400 FYT⁽²⁾

1000 FYT⁽²⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500 FYT ⁽²⁾.

3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.

30.6.2004 ^(I)(c)

Chickens for fattening

-

200 FYT⁽²⁾

1000 FYT⁽²⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 500 FYT ⁽²⁾.

3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.

30.6.2004 ^(I)(c)

3

Alpha-galactosidase
EC 3.2.1.22

Preparation of alpha-galactosidase produced by Aspergillus oryzae (DSM 10 286) having a minimum activity of;
Liquid form: 1000 GALU⁽³⁾/g

Chicken for fattening

-

300 GALU⁽³⁾

1000 GALU⁽³⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 450 GALU ⁽³⁾.

3. For use in compound feed rich in oligosaccharides, e.g. containing more than 25% soy meal, cotton seed cakes, peas.

30.6.2004 ^(I)(c)

4

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
Coated form: 50 FBG⁽⁴⁾/g
Liquid form: 120 FBG⁽⁴⁾/g

Piglets

Four months

25 FBG⁽⁴⁾

40 FBG⁽⁴⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 25 FBG⁽⁴⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50 % corn or barley.

30.6.2004 ^(I)(c)

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
Coated form: 50 FBG^(4')/g
Liquid form: 120 FBG^(4')/g

Chicken for fattening

-

Endo-1,3(4)-
beta-glucanase:
10 FBG^(4')

Endo-1,3(4)-
beta-glucanase:
100 FBG^(4')

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 20 FBG^(4').

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 60 % maize.

1.4.2004 ^(I)(d)

5

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Aspergillus oryzae (DSM 10287) having a minimum activity of:
Coated form: 1000 FXU⁽⁵⁾/g
Liquid form: 650 FXU⁽⁵⁾/ml

Chickens for fattening

-

80 FXU⁽⁵⁾

200 FXU⁽⁵⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 150 FXU⁽⁵⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat.

30.6.2004 ^(I)(c)

Turkeys for fattening

-

225 FXU⁽⁵⁾

600 FXU⁽⁵⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 225 to 600 FXU⁽⁵⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat.

30.6.2004 ^(I)(c)

Piglets

Four months

200 FXU⁽⁵⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 200 FXU⁽⁵⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat.

30.6.2004 ^(I)(c)

6

Endo-1,4-beta-
xylanase EC 3.2.1.8

Endo-1,4-beta-
glucanase EC 3.2.1.4

Preparation of endo-1,4-beta-xylanase and endo-1,4-beta-
glucanase produced by Humicola insolens (DSM 10442) having a minimum activity of:

Coated form: 800 FXU⁽⁶⁾/g75 FBG⁽⁷⁾/g

Microgranulated form: 800 FXU⁽⁶⁾/g
75 FBG⁽⁷⁾/g

Liquid form: 550 FXU⁽⁶⁾ /g
50 FBG⁽⁷⁾/g

Pigs for fattening

-

200 FXU⁽⁶⁾
19 FBG⁽⁷⁾

800 FXU⁽⁶⁾
75 FBG⁽⁷⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

400 FXU⁽⁶⁾,
38 FBG⁽⁷⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley, and/or oats, wheat.

30.6.2004 ^(I)(e)

Chickens for fattening

-

200 FXU⁽⁶⁾
19 FBG⁽⁷⁾

1000 FXU⁽⁶⁾
94 FBG⁽⁷⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

400 FXU⁽⁶⁾,
38 FBG⁽⁷⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley, and/or oats, wheat.

30.6.2004 ^(I)(c)

Piglets

Four months

240 FXU⁽⁶⁾
22 FBG⁽⁷⁾

1000 FXU⁽⁶⁾
94 FBG⁽⁷⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

400 FXU⁽⁶⁾,
38 FBG⁽⁷⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley, and/or oats, wheat.

30.6.2004 ^(I)(c)

7

Endo-1,4-beta-
xylanase EC 3.2.1.8

Endo-1,4-beta-
glucanase EC 3.2.1.4

Preparation of endo -1,4-beta-xylanase and endo -1,4-beta-
glucanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of:

Coated form:
36000 FXU⁽⁸⁾ /g
15000 BGU⁽⁹⁾/g

Liquid form:
36000 FXU⁽⁸⁾ /g
15000 BGU⁽⁹⁾/g

Chickens for fattening

-

3600 FXU⁽⁸⁾

1500 BGU⁽⁹⁾

12000 FXU⁽⁸⁾

5000 BGU⁽⁹⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

3600 to 6000 FXU⁽⁸⁾,
1500 to 2500 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 35% barley and 20% wheat.

1.4.2004 ^(I)(c)

Piglets

Four months

6000 FXU⁽⁸⁾

2500 BGU⁽⁹⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

6000 FXU⁽⁸⁾,
2500 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley and 30% wheat.

1.4.2004 ^(I)(d)

Turkeys for fattening

-

6000 FXU⁽⁸⁾

2500 BGU⁽⁹⁾

12000 FXU⁽⁸⁾

5000 BGU⁽⁹⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

6000 to 12000 FXU⁽⁸⁾,
2500 to 5000 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% wheat.

1.4.2004 ^(I)(d)

Laying hens

-

12000 FXU⁽⁸⁾

5000 BGU⁽⁹⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

Endo-1,4-beta-xylanase:
12000 FXU⁽⁸⁾,

Endo-1,4-beta-glucanase:
5000 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 10% barley, 20% sunflower and 20% wheat.

1.4.2004 ^(I)(d)

Preparation of endo -1,4-beta-xylanase and endo -1,4-beta-
glucanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of:

Solid form:
36000 FXU⁽⁸⁾ /g
15000 BGU⁽⁹⁾/g

Chickens for fattening

-

3600 FXU⁽⁸⁾

1500 BGU⁽⁹⁾

12000 FXU⁽⁸⁾

5000 BGU⁽⁹⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

3600 to 6000 FXU⁽⁸⁾,
1500 to 2500 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 35% barley and 20% wheat.

30.9.2004 ^(I)(h)

Piglets

Four months

6000 FXU⁽⁸⁾

2500 BGU⁽⁹⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

6000 FXU⁽⁸⁾,
2500 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley and 30% wheat.

30.9.2004 ^(I)(h)

Turkeys for fattening

-

6000 FXU⁽⁸⁾

2500 BGU⁽⁹⁾

12000 FXU⁽⁸⁾

5000 BGU⁽⁹⁾

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

6000 to 12000 FXU⁽⁸⁾,
2500 to 5000 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% wheat.

30.9.2004 ^(I)(h)

Laying hens

-

12000 FXU⁽⁸⁾

5000 BGU⁽⁹⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

Endo-1,4-beta-xylanase:
12000 FXU⁽⁸⁾,

Endo-1,4-beta-glucanase:
5000 BGU⁽⁹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 10% barley, 20% sunflower and 20% wheat.

30.9.2004 ^(I)(h)

8

Endo-1,4-beta-
glucanase EC 3.2.1.4

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo -1,
4-beta-glucanase and endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of:

Coated form:
10000 BGU⁽¹⁰⁾/g
4000 FXU⁽¹¹⁾/g

Liquid form:
20000 BGU⁽¹⁰⁾/g
8000 FXU⁽¹¹⁾/g

Piglets

Four months

3000 BGU⁽¹⁰⁾

1200 FXU⁽¹¹⁾

5000 BGU⁽¹⁰⁾

2000 FXU⁽¹¹⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

3000 to 5000 BGU⁽¹⁰⁾1200 to 2000 FXU⁽¹¹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% barley.

1.4.2004 ^(I)(d)

Chickens for fattening

-

3000 BGU⁽¹⁰⁾

1200 FXU⁽¹¹⁾

10000 BGU⁽¹⁰⁾

4000 FXU⁽¹¹⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

3000 to 10000 BGU⁽¹⁰⁾1200 to 4000 FXU⁽¹¹⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.

1.4.2004 ^(I)(c)

Laying hens

-

5000 BGU⁽¹⁰⁾

2000 FXU⁽¹¹⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

5000 BGU⁽¹⁰⁾2000 FXU⁽¹¹⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.

1.4.2004 ^(I)(d)

Preparation of endo -1,
4-beta-glucanase and endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of:

Solid form:
20000 BGU⁽¹⁰⁾/g
8000 FXU⁽¹¹⁾/g

Piglets

Four months

3000 BGU⁽¹⁰⁾

1200 FXU⁽¹¹⁾

5000 BGU⁽¹⁰⁾

2000 FXU⁽¹¹⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

3000 to 5000 BGU⁽¹⁰⁾1200 to 2000 FXU⁽¹¹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% barley.

30.9.2004 ^(I)(h)

Chickens for fattening

-

3000 BGU⁽¹⁰⁾

1200 FXU⁽¹¹⁾

10000 BGU⁽¹⁰⁾

4000 FXU⁽¹¹⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

3000 to 10000 BGU⁽¹⁰⁾1200 to 4000 FXU⁽¹¹⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.

30.9.2004 ^(I)(h)

Laying hens

-

5000 BGU⁽¹⁰⁾

2000 FXU⁽¹¹⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

5000 BGU⁽¹⁰⁾2000 FXU⁽¹¹⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.

30.9.2004 ^(I)(h)

9

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 270.95) having a minimum activity of:

Solid form:
28000 EXU⁽¹²⁾/g

Liquid form:
28000 EXU⁽¹²⁾/ml

Chickens for fattening

-

1400 EXU⁽¹²⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1400 EXU⁽¹²⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50% wheat.

30.6.2004 ^(I)(c)

Preparation of endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 270.95) having a minimum activity of:

Solid form:
Endo-1,4-beta-xylanase:
28000 EXU⁽¹²⁾/g

Liquid form:
Endo-1,4-beta-xylanase:
14000 EXU⁽¹²⁾/ml

Laying hens

-

2400 EXU⁽¹²⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

2400 to 7400 EXU⁽¹²⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 30% wheat and 30% rye.

30.6.2004 ^(I)(d)

Turkeys for fattening

-

2400 EXU⁽¹²⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

2400 to 5600 EXU⁽¹²⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 30% wheat and 30% rye.

30.6.2004 ^(I)(d)

10

Alpha-amylase
EC 3.2.1.1.

Preparation of alpha-amylase produced by Bacillus amyloliquefaciens (CBS 360.94) having a minimum activity of:

Solid form: 45000 RAU⁽¹³⁾/g
Liquid form: 20000 RAU⁽¹³⁾/ml

Piglets

Four months

1800 RAU⁽¹³⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1800 RAU⁽¹³⁾.

3. For use, exclusively, in compound feed destined for liquid feeding systems, and containing strach-rich feed materials (e.g. containing more than 35% wheat).

30.6.2004 ^(I)(c)

Pigs for fattening

-

1800 RAU⁽¹³⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1800 RAU⁽¹³⁾.

3. For use, exclusively, in compound feed destined for liquid feeding systems, and containing strach-rich feed materials (e.g. containing more than 35% wheat).

30.6.2004 ^(I)(c)

Sows

-

1800 RAU⁽¹³⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 1800 RAU⁽¹³⁾.

3. For use, exclusively, in compound feed destined for liquid feeding systems, and containing strach-rich feed materials (e.g. containing more than 35% wheat).

30.6.2004 ^(I)(c)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)

(a) First authorisation Commission Regulation (EC) N° 2316/98 (OJ L 289, 28.10.1998)
(b) First authorisation Commission Regulation (EC) N° 866/1999 (OJ L 108, 27.4.1999)
(c) First authorisation Commission Regulation (EC) N° 1436/98 (OJ L 191, 7.7.1998)
(d) First authorisation Commission Regulation (EC) N° 654/2000 (OJ L 79, 30.3.2000)
(e) First authorisation Commission Regulation (EC) N° 1411/1999 (OJ L 164, 30.6.1999)
(h) First authorisation Commission Regulation (EC) N° 2697/2000 (OJ L 319, 16.12.2000)

(1) FTU is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute fom sodium phytate at pH 5,5 and 37°C.
(2) FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5,5 and 37°C.
(3) 1 GALU is the amount of enzyme which hydrolyses 1 micromole of p-nitrophenyl-alpha-galactopyranoside per minute at pH 5 and 30°C.
(4) 1 FBG is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,5 and 37°C.
(4') 1 FBG is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(5) 1 FXU is the amount of enzyme which liberates 7,8 micromoles of reducing sugars (xylose equivalents) from azo-wheat arabinoxylan per minute at pH 6,0 and 50°C.
(6) 1 FXU is the amount of enzyme which liberates 3,1 micromoles of reducing sugars (xylose equivalents) from azo-wheat arabinoxylan per minute at pH 6,0 and 50°C.
(7) 1 FBG is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 6,0 and 50°C.
(8) 1 FXU is the amount of enzyme which liberates 0,15 micromoles of xylose from azurine-cross-linked xylan per minute at pH 5,0 and 40°C.
(9) 1 BGU is the amount of enzyme which liberates 0,15 micromoles of glucose from azurine-cross-linked beta-glucan per minute at pH 5,0 and 40°C.
(10) 1 BGU is the amount of enzyme which liberates 0,15 micromoles of glucose from azurine-cross-linked beta-glucan per minute at pH 5,0 and 40°C.
(11) 1 FXU is the amount of enzyme which liberates 0,15 micromoles of xylose from azurine-cross-linked xylan per minute at pH 5,0 and 40°C.
(12) 1 EXU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from arabinoxylan per minute at pH 3,5 and 55°C.
(13) 1 RAU is the amount of enzyme which converts 1 mg of soluble starch into a product having an equal absorption to a reference colour at 620 nm after reaction with iodine, per minute at pH 6,6 and 30°C.

[back to Directive text]

EC N°	Additive	Chemical formula, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

E 1600	3-phytase (EC 3.1.3.8)	Preparation of 3-phytase produced by Aspergillus niger (CBS 114.94) having a minimum phytase activity of: Solid form: 5000 FTU ⁽¹⁾/g Liquid form: 5000 FTU ⁽¹⁾/ml	Piglets	2 months	500 FTU⁽¹⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 500 FTU⁽¹⁾. 3. For use in compound feed containind more than 0,23% phytin bound phosphorus	Without a time limit
			Pigs for fattening	-	280 FTU⁽¹⁾	^-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 400-500 FTU⁽¹⁾. 3. For use in compound feed containind more than 0,23% phytin bound phosphorus	Without a time limit
			Sows	-	500 FTU⁽¹⁾	^-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 500 FTU⁽¹⁾. 3. For use in compound feed containind more than 0,36% phytin bound phosphorus	Without a time limit
			Chickens for fattening	-	375 FTU⁽¹⁾	^-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 500-700 FTU⁽¹⁾. 3. For use in compound feed containind more than 0,23% phytin bound phosphorus	Without a time limit
			Laying hens	-	250 FTU⁽¹⁾	^-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 300-400 FTU⁽¹⁾. 3. For use in compound feed containind more than 0,23% phytin bound phosphorus	Without a time limit

N°	Additive	Chemical name, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

1	3-phytase (EC 3.1.3.8)	Preparation of 3-phytase produced by Aspergillus niger (CBS 114.94) having a minimum phytase activity of 5000 FTU ⁽¹⁾/g for solid and liquid preparations	Turkeys	-	125 FTU⁽¹⁾	-	1. Indicate in the directions for use for the additive and the premixture the storage temperature, the storage duration and stability on pelleting. 2. Recommended dose per kg of complete feedingstuff: 200 to 800 FTU⁽¹⁾. 3. For use in compound feedingstuffs with a minimum content of 0,3% phytase, e.g. 20% wheat.	14.12.2003 ^(I)(a)
2	3-phytase (EC 3.1.3.8)	Preparation of 3-phytase produced by Aspergillus oryzae (DMS 10 289) having a minimum activity of: Coated form: 2500 FYT⁽²⁾/g Liquid form: 5000 FYT⁽²⁾/g	Laying hens	-	500 FYT ⁽²⁾	1000 FYT⁽²⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 750 FYT ⁽²⁾. 3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.	30.6.2004 ^(I)(b)
			Piglets	Four months	250 FYT⁽²⁾	1000 FYT⁽²⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 500 FYT ⁽²⁾. 3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.	30.6.2004 ^(I)(c)
			Pigs for fattening	-	400 FYT⁽²⁾	1000 FYT⁽²⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 500 FYT ⁽²⁾. 3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.	30.6.2004 ^(I)(c)
			Chickens for fattening	-	200 FYT⁽²⁾	1000 FYT⁽²⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 500 FYT ⁽²⁾. 3. For use in compound feed rich in phytates, e.g. containing more than 40% cereals (corn, barley, oats, wheat, rye, triticale), oilseeds and pulses.	30.6.2004 ^(I)(c)
3	Alpha-galactosidase EC 3.2.1.22	Preparation of alpha-galactosidase produced by Aspergillus oryzae (DSM 10 286) having a minimum activity of; Liquid form: 1000 GALU⁽³⁾/g	Chicken for fattening	-	300 GALU⁽³⁾	1000 GALU⁽³⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 450 GALU ⁽³⁾. 3. For use in compound feed rich in oligosaccharides, e.g. containing more than 25% soy meal, cotton seed cakes, peas.	30.6.2004 ^(I)(c)
4	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: Coated form: 50 FBG⁽⁴⁾/g Liquid form: 120 FBG⁽⁴⁾/g	Piglets	Four months	25 FBG⁽⁴⁾	40 FBG⁽⁴⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 25 FBG⁽⁴⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50 % corn or barley.	30.6.2004 ^(I)(c)
4	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: Coated form: 50 FBG^(4')/g Liquid form: 120 FBG^(4')/g	Chicken for fattening	-	Endo-1,3(4)- beta-glucanase: 10 FBG^(4')	Endo-1,3(4)- beta-glucanase: 100 FBG^(4')	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 20 FBG^(4'). 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 60 % maize.	1.4.2004 ^(I)(d)
5	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Aspergillus oryzae (DSM 10287) having a minimum activity of: Coated form: 1000 FXU⁽⁵⁾/g Liquid form: 650 FXU⁽⁵⁾/ml	Chickens for fattening	-	80 FXU⁽⁵⁾	200 FXU⁽⁵⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 150 FXU⁽⁵⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat.	30.6.2004 ^(I)(c)
			Turkeys for fattening	-	225 FXU⁽⁵⁾	600 FXU⁽⁵⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 225 to 600 FXU⁽⁵⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat.	30.6.2004 ^(I)(c)
			Piglets	Four months	200 FXU⁽⁵⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 200 FXU⁽⁵⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50 % wheat.	30.6.2004 ^(I)(c)
6	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,4-beta- glucanase EC 3.2.1.4	Preparation of endo-1,4-beta-xylanase and endo-1,4-beta- glucanase produced by Humicola insolens (DSM 10442) having a minimum activity of: Coated form: 800 FXU⁽⁶⁾/g75 FBG⁽⁷⁾/g Microgranulated form: 800 FXU⁽⁶⁾/g 75 FBG⁽⁷⁾/g Liquid form: 550 FXU⁽⁶⁾ /g 50 FBG⁽⁷⁾/g	Pigs for fattening	-	200 FXU⁽⁶⁾ 19 FBG⁽⁷⁾	800 FXU⁽⁶⁾ 75 FBG⁽⁷⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 400 FXU⁽⁶⁾, 38 FBG⁽⁷⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley, and/or oats, wheat.	30.6.2004 ^(I)(e)
			Chickens for fattening	-	200 FXU⁽⁶⁾ 19 FBG⁽⁷⁾	1000 FXU⁽⁶⁾ 94 FBG⁽⁷⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 400 FXU⁽⁶⁾, 38 FBG⁽⁷⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley, and/or oats, wheat.	30.6.2004 ^(I)(c)
			Piglets	Four months	240 FXU⁽⁶⁾ 22 FBG⁽⁷⁾	1000 FXU⁽⁶⁾ 94 FBG⁽⁷⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 400 FXU⁽⁶⁾, 38 FBG⁽⁷⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley, and/or oats, wheat.	30.6.2004 ^(I)(c)
7	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,4-beta- glucanase EC 3.2.1.4	Preparation of endo -1,4-beta-xylanase and endo -1,4-beta- glucanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of: Coated form: 36000 FXU⁽⁸⁾ /g 15000 BGU⁽⁹⁾/g Liquid form: 36000 FXU⁽⁸⁾ /g 15000 BGU⁽⁹⁾/g	Chickens for fattening	-	3600 FXU⁽⁸⁾ 1500 BGU⁽⁹⁾	12000 FXU⁽⁸⁾ 5000 BGU⁽⁹⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 3600 to 6000 FXU⁽⁸⁾, 1500 to 2500 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 35% barley and 20% wheat.	1.4.2004 ^(I)(c)
			Piglets	Four months	6000 FXU⁽⁸⁾ 2500 BGU⁽⁹⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 6000 FXU⁽⁸⁾, 2500 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley and 30% wheat.	1.4.2004 ^(I)(d)
			Turkeys for fattening	-	6000 FXU⁽⁸⁾ 2500 BGU⁽⁹⁾	12000 FXU⁽⁸⁾ 5000 BGU⁽⁹⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 6000 to 12000 FXU⁽⁸⁾, 2500 to 5000 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% wheat.	1.4.2004 ^(I)(d)
			Laying hens	-	12000 FXU⁽⁸⁾ 5000 BGU⁽⁹⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: Endo-1,4-beta-xylanase: 12000 FXU⁽⁸⁾, Endo-1,4-beta-glucanase: 5000 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 10% barley, 20% sunflower and 20% wheat.	1.4.2004 ^(I)(d)
		Preparation of endo -1,4-beta-xylanase and endo -1,4-beta- glucanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of: Solid form: 36000 FXU⁽⁸⁾ /g 15000 BGU⁽⁹⁾/g	Chickens for fattening	-	3600 FXU⁽⁸⁾ 1500 BGU⁽⁹⁾	12000 FXU⁽⁸⁾ 5000 BGU⁽⁹⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 3600 to 6000 FXU⁽⁸⁾, 1500 to 2500 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 35% barley and 20% wheat.	30.9.2004 ^(I)(h)
			Piglets	Four months	6000 FXU⁽⁸⁾ 2500 BGU⁽⁹⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 6000 FXU⁽⁸⁾, 2500 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% barley and 30% wheat.	30.9.2004 ^(I)(h)
			Turkeys for fattening	-	6000 FXU⁽⁸⁾ 2500 BGU⁽⁹⁾	12000 FXU⁽⁸⁾ 5000 BGU⁽⁹⁾	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 6000 to 12000 FXU⁽⁸⁾, 2500 to 5000 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% wheat.	30.9.2004 ^(I)(h)
			Laying hens	-	12000 FXU⁽⁸⁾ 5000 BGU⁽⁹⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: Endo-1,4-beta-xylanase: 12000 FXU⁽⁸⁾, Endo-1,4-beta-glucanase: 5000 BGU⁽⁹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 10% barley, 20% sunflower and 20% wheat.	30.9.2004 ^(I)(h)
8	Endo-1,4-beta- glucanase EC 3.2.1.4 Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1, 4-beta-glucanase and endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of: Coated form: 10000 BGU⁽¹⁰⁾/g 4000 FXU⁽¹¹⁾/g Liquid form: 20000 BGU⁽¹⁰⁾/g 8000 FXU⁽¹¹⁾/g	Piglets	Four months	3000 BGU⁽¹⁰⁾ 1200 FXU⁽¹¹⁾	5000 BGU⁽¹⁰⁾ 2000 FXU⁽¹¹⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 3000 to 5000 BGU⁽¹⁰⁾1200 to 2000 FXU⁽¹¹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% barley.	1.4.2004 ^(I)(d)
			Chickens for fattening	-	3000 BGU⁽¹⁰⁾ 1200 FXU⁽¹¹⁾	10000 BGU⁽¹⁰⁾ 4000 FXU⁽¹¹⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 3000 to 10000 BGU⁽¹⁰⁾1200 to 4000 FXU⁽¹¹⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.	1.4.2004 ^(I)(c)
			Laying hens	-	5000 BGU⁽¹⁰⁾ 2000 FXU⁽¹¹⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 5000 BGU⁽¹⁰⁾2000 FXU⁽¹¹⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.	1.4.2004 ^(I)(d)
		Preparation of endo -1, 4-beta-glucanase and endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 600.94) having a minimum activity of: Solid form: 20000 BGU⁽¹⁰⁾/g 8000 FXU⁽¹¹⁾/g	Piglets	Four months	3000 BGU⁽¹⁰⁾ 1200 FXU⁽¹¹⁾	5000 BGU⁽¹⁰⁾ 2000 FXU⁽¹¹⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 3000 to 5000 BGU⁽¹⁰⁾1200 to 2000 FXU⁽¹¹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% barley.	30.9.2004 ^(I)(h)
			Chickens for fattening	-	3000 BGU⁽¹⁰⁾ 1200 FXU⁽¹¹⁾	10000 BGU⁽¹⁰⁾ 4000 FXU⁽¹¹⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 3000 to 10000 BGU⁽¹⁰⁾1200 to 4000 FXU⁽¹¹⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.	30.9.2004 ^(I)(h)
			Laying hens	-	5000 BGU⁽¹⁰⁾ 2000 FXU⁽¹¹⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 5000 BGU⁽¹⁰⁾2000 FXU⁽¹¹⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.	30.9.2004 ^(I)(h)
9	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 270.95) having a minimum activity of: Solid form: 28000 EXU⁽¹²⁾/g Liquid form: 28000 EXU⁽¹²⁾/ml	Chickens for fattening	-	1400 EXU⁽¹²⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 1400 EXU⁽¹²⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50% wheat.	30.6.2004 ^(I)(c)
		Preparation of endo -1,4-beta-xylanase produced by Aspergillus niger (CBS 270.95) having a minimum activity of: Solid form: Endo-1,4-beta-xylanase: 28000 EXU⁽¹²⁾/g Liquid form: Endo-1,4-beta-xylanase: 14000 EXU⁽¹²⁾/ml	Laying hens	-	2400 EXU⁽¹²⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 2400 to 7400 EXU⁽¹²⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 30% wheat and 30% rye.	30.6.2004 ^(I)(d)
			Turkeys for fattening	-	2400 EXU⁽¹²⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 2400 to 5600 EXU⁽¹²⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 30% wheat and 30% rye.	30.6.2004 ^(I)(d)
10	Alpha-amylase EC 3.2.1.1.	Preparation of alpha-amylase produced by Bacillus amyloliquefaciens (CBS 360.94) having a minimum activity of: Solid form: 45000 RAU⁽¹³⁾/g Liquid form: 20000 RAU⁽¹³⁾/ml	Piglets	Four months	1800 RAU⁽¹³⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 1800 RAU⁽¹³⁾. 3. For use, exclusively, in compound feed destined for liquid feeding systems, and containing strach-rich feed materials (e.g. containing more than 35% wheat).	30.6.2004 ^(I)(c)
			Pigs for fattening	-	1800 RAU⁽¹³⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 1800 RAU⁽¹³⁾. 3. For use, exclusively, in compound feed destined for liquid feeding systems, and containing strach-rich feed materials (e.g. containing more than 35% wheat).	30.6.2004 ^(I)(c)
			Sows	-	1800 RAU⁽¹³⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 1800 RAU⁽¹³⁾. 3. For use, exclusively, in compound feed destined for liquid feeding systems, and containing strach-rich feed materials (e.g. containing more than 35% wheat).	30.6.2004 ^(I)(c)