70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (2)

N°
Additive Chemical formula, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

11

Endo-1,4-beta-
glucanase EC 3.2.1.4

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo -1,
4-beta- glucanase, endo -1,3(4)-beta- glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 74 252) having a minimum activity of:

Endo-1,4-beta-
glucanase: 8000 U⁽¹⁾/ml

Endo-1,3(4)-beta-
glucanase : 18000 U⁽²⁾/ml

Endo-1,4-beta-xylanase : 26000 U⁽³⁾/ml

Chickens for fattening

-

Endo-1,4-beta-
glucanase: 400 U⁽¹⁾

Endo-1,3(4)-
beta-glucanase : 900 U⁽²⁾

Endo-1,4-beta-
xylanase : 1300 U⁽³⁾

^-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

endo-1,4-beta-
glucanase: 400 to 1600 U⁽¹⁾,
endo-1,3(4)-beta-
glucanase : 900 to 3600 U⁽²⁾,
endo-1,4-beta-xylanase : 1300 to 5200 U⁽³⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% wheat or barley and 10% rye.

30.6.2004 ^(I)(a)

Turkeys for fattening

-

Endo-1,4-beta-
glucanase: 400 U⁽¹⁾

Endo-1,3(4)-
beta-glucanase : 900 U⁽²⁾

Endo-1,4-beta-
xylanase : 1300 U⁽³⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

endo-1,4-beta-
glucanase: 400 to 800 U⁽¹⁾,
endo-1,3(4)-beta-
glucanase : 900 to 1800 U⁽²⁾,
endo-1,4-beta-xylanase : 1300 to 2600 U⁽³⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% wheat.

31.05.2005 ^(I)(b)

12

Endo-1,4-beta-
glucanase EC 3.2.1.4

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo -1,
4-beta- glucanase, endo -1,3(4)-beta- glucanase and endo -1,4-beta-xylanase produced by Trichoderma viride (FERM BP-4447) having a minimum activity of:

Endo-1,4-beta-glucanase: 8000 U⁽⁴⁾/g

Endo-1,3(4)-beta-
glucanase : 18000 U⁽⁵⁾/g

Endo-1,4-beta-xylanase : 26000 U⁽⁶⁾/g

Chickens for fattening

-

Endo-1,4-beta-
glucanase: 200 U⁽⁴⁾

Endo-1,3(4)-
beta-glucanase : 450 U⁽⁵⁾

Endo-1,4-beta-
xylanase : 650 U⁽⁶⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

endo-1,4-beta-
glucanase: 800 to 1200 U⁽⁴⁾,endo-1,3(4)-beta-
glucanase : 1800 to 2700 U⁽⁵⁾,endo-1,4-beta-xylanase : 2600 to 3900 U⁽⁶⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 20% barley and/or 25% rye.

30.6.2004 ^(I)(a)

Laying hens

-

Endo-1,4-beta-
glucanase: 640 U⁽³⁾

Endo-1,3(4)-
beta-glucanase : 1440 U⁽⁵⁾

Endo-1,4-beta-
xylanase : 2080 U⁽⁶⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff:

endo-1,4-beta-
glucanase: 640 to 1280 U⁽⁴⁾,endo-1,3(4)-beta-
glucanase : 1440 to 2880 U⁽⁵⁾,endo-1,4-beta-xylanase : 2080 to 4160 U⁽⁶⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 20% barley and/or 25% rye.

30.6.2004 ^(I)(a)

Turkeys for fattening

-

Endo-1,4-beta-
glucanase: 800 U⁽⁴⁾

Endo-1,3(4)-
beta-glucanase : 1800 U⁽⁵⁾

Endo-1,4-beta-
xylanase : 2600 U⁽⁶⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-
glucanase: 800 to 1200 U⁽⁴⁾,endo-1,3(4)-beta-
glucanase : 1800 to 2700 U⁽⁵⁾,endo-1,4-beta-xylanase : 2600 to 3900 U⁽⁶⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 20% barley.

30.6.2004 ^(I)(a)

13

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo -1,3(4)-beta- glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CBS 357.94) having a minimum activity of:

Powder form:
Endo-1,3(4)-beta-
glucanase:
8000 BGU⁽⁷⁾/g
Endo-1,4-beta-xylanase:
11000 EXU⁽⁸⁾/g

Granulated form:
Endo-1,3(4)-beta-
glucanase:
6000 BGU⁽⁷⁾/g
Endo-1,4-beta-xylanase:
8250 EXU⁽⁸⁾/g

Liquid form:
Endo-1,3(4)-beta-
glucanase
2000 BGU⁽⁷⁾/ml
Endo-1,4-beta-
xylanase:
2750 EXU⁽⁸⁾/ml

Chickens for fattening

-

100 BGU⁽⁷⁾
130 EXU⁽⁸⁾

^-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

100 BGU⁽⁷⁾,
130 EXU⁽⁸⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% wheat and 30% barley or 20% rye.

30.6.2004 ^(I)(a)

Laying hens

-

600 BGU⁽⁷⁾

800 EXU⁽⁸⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

600 BGU⁽⁷⁾,
800 EXU⁽⁸⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40% wheat and more than 30% barley.

30.6.2004 ^(I)(d)

Turkeys for fattening

-

600 BGU⁽⁷⁾

800 EXU⁽⁸⁾

-

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff:

600 BGU⁽⁷⁾,
800 EXU⁽⁸⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% wheat or more than 30% rye.

30.6.2004 ^(I)(d)

14

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 520.94) having minimum activities of:

Solid form: 600 U⁽⁹⁾/g

Liquid form: 300 U⁽⁹⁾/ml

Chickens for fattening

-

Endo-1,4-beta-
xylanase: 300 U⁽⁹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 300 to 600 U⁽⁹⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50% wheat.

30.6.2004 ^(I)(a)

15

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Preparation of endo -1,
3(4)-beta- glucanase produced by Trichoderma viride (CBS 517.94) having a minimum activity of:

Solid form: 650 U⁽¹⁰⁾/g

Liquid form: 325 U⁽¹⁰⁾/ml

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 325 U⁽¹⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 325 to 650 U⁽¹⁰⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50% barley.

30.6.2004 ^(I)(a)

16

Endo-1,4-beta-
glucanase EC 3.2.1.4

Preparation of endo-1,
4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of:

Liquid form: 2000 CU⁽¹¹⁾/ml

Chickens for fattening

-

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

30.6.2004 ^(I)(a)

Laying hens

-

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

30.6.2004 ^(I)(a)

Piglets

Four months

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

30.6.2004 ^(I)(a)

Pigs for fattening

-

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

30.6.2004 ^(I)(a)

Preparation of endo-1,
4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of:

Solid form: 2000 CU⁽¹¹⁾/g
Liquid form: 2000 CU⁽¹¹⁾/ml

Chickens for fattening

-

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

17.7.2004 ^(I)(c)

Laying hens

-

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

17.7.2004 ^(I)(c)

Piglets

Four months

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

17.7.2004 ^(I)(c)

Pigs for fattening

-

250 CU⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.

17.7.2004 ^(I)(c)

17

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:

Liquid form: 2000 EPU⁽¹²⁾/ml

Chickens for fattening

-

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

30.6.2004 ^(I)(a)

Laying hens

-

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

30.6.2004 ^(I)(a)

Piglets

Four months

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

30.6.2004 ^(I)(a)

Pigs for fattening

-

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

30.6.2004 ^(I)(a)

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:

Solid form: 6000 EPU⁽¹²⁾/g

Chickens for fattening

-

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

14.7.2004 ^(I)(c)

Laying hens

-

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

14.7.2004 ^(I)(c)

Piglets

Four months

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

14.7.2004 ^(I)(c)

Pigs for fattening

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.

14.7.2004 ^(I)(c)

Turkeys for fattening

-

750 EPU⁽¹²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 35% wheat or maize.

14.7.2004 ^(I)(c)

18

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus niger (MUCL 39199) having a minimum activity of:

Solid form: 2000 AGL⁽¹³⁾/g
Liquid form: 500 AGL⁽¹³⁾/ml

Chickens for fattening

-

100 AGL⁽¹³⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 100 AGL⁽¹³⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glycans) e.g. containing more than 40% barley and 20% wheat.

30.6.2004 ^(I)(a)

19

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus niger (MUCL 39199) having a minimum activity of:

Solid form: 1500 AGL⁽¹⁴⁾/g
Liquid form: 200 AGL⁽¹⁴⁾/g

Chickens for fattening

-

25 AGL⁽¹⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 25 to 100 AGL⁽¹⁴⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 50% barley.

30.6.2004 ^(I)(a)

20

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (MUCL 39203) having a minimum activity of:

Solid form: 2000 AXC⁽¹⁵⁾/g
Liquid form: 500 AXC⁽¹⁵⁾/ml

Chicken for fattening

-

100 AXC⁽¹⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 100 AXC⁽¹⁵⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 40% wheat or rye.

30.6.2004 ^(I)(a)

21

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (MUCL 39203) having a minimum activity of:

Solid form: 1500 AXC⁽¹⁶⁾/g
Liquid form: 200 AXC⁽¹⁶⁾/g

Chickens for fattening

-

25 AXC⁽¹⁶⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 25 to 100 AXC⁽¹⁶⁾.

3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50% wheat.

30.6.2004 ^(II)(a)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)

(a) First authorisation Commission Regulation (EC) N° 1436/98 (OJ L 191, 7.7.1998)
(b) First authorisation Commission Regulation (EC) N° 937/2001 (OJ L 130, 12.5.2001)
(c) First authorisation Commission Regulation (EC) N° 1353/2000 (OJ L 155, 28.6.2000)

(1) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40°C.
(2) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40°C.
(3) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40°C.
(4) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40°C.
(5) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40°C.
(6) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40°C.
(7) 1 BGU is the amount of enzyme which liberates 0,278 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 3,5 and 40°C.
(8) 1 EXU is the amount of enzyme which liberates 1 micromoles of reducing sugars (xylose equivalents) from wheat arabinoxylan per minute at pH 3,5 and 55°C.
(9) 1 U is the amount of enzyme which liberates 1 micromoles of xylose from birchwood xylan per minute at pH 5,3 and 50°C.
(10) 1 U is the amount of enzyme which liberates 1 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(11) 1 CU is the amount of enzyme which liberates 0,128 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,5 and 30°C.
(12) 1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30°C.
(13) 1 AGL is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 4,6 and 30°C.
(14) 1 AGL is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 4,6 and 30°C.
(15) 1 AGL is the amount of enzyme which liberates 17,2 micromoles of reducing sugars (maltose equivalents) from oat xylan per minute at pH 4,7 and 30°C.
(16) 1 AGL is the amount of enzyme which liberates 17,2 micromoles of reducing sugars (maltose equivalents) from oat xylan per minute at pH 4,7 and 30°C.

[back to Directive text]

N°	Additive	Chemical formula, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

11	Endo-1,4-beta- glucanase EC 3.2.1.4 Endo-1,3(4)-beta- glucanase EC 3.2.1.6 Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1, 4-beta- glucanase, endo -1,3(4)-beta- glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 74 252) having a minimum activity of: Endo-1,4-beta- glucanase: 8000 U⁽¹⁾/ml Endo-1,3(4)-beta- glucanase : 18000 U⁽²⁾/ml Endo-1,4-beta-xylanase : 26000 U⁽³⁾/ml	Chickens for fattening	-	Endo-1,4-beta- glucanase: 400 U⁽¹⁾ Endo-1,3(4)- beta-glucanase : 900 U⁽²⁾ Endo-1,4-beta- xylanase : 1300 U⁽³⁾	^-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: endo-1,4-beta- glucanase: 400 to 1600 U⁽¹⁾, endo-1,3(4)-beta- glucanase : 900 to 3600 U⁽²⁾, endo-1,4-beta-xylanase : 1300 to 5200 U⁽³⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% wheat or barley and 10% rye.	30.6.2004 ^(I)(a)
11			Turkeys for fattening	-	Endo-1,4-beta- glucanase: 400 U⁽¹⁾ Endo-1,3(4)- beta-glucanase : 900 U⁽²⁾ Endo-1,4-beta- xylanase : 1300 U⁽³⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: endo-1,4-beta- glucanase: 400 to 800 U⁽¹⁾, endo-1,3(4)-beta- glucanase : 900 to 1800 U⁽²⁾, endo-1,4-beta-xylanase : 1300 to 2600 U⁽³⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% wheat.	31.05.2005 ^(I)(b)
12	Endo-1,4-beta- glucanase EC 3.2.1.4 Endo-1,3(4)-beta- glucanase EC 3.2.1.6 Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1, 4-beta- glucanase, endo -1,3(4)-beta- glucanase and endo -1,4-beta-xylanase produced by Trichoderma viride (FERM BP-4447) having a minimum activity of: Endo-1,4-beta-glucanase: 8000 U⁽⁴⁾/g Endo-1,3(4)-beta- glucanase : 18000 U⁽⁵⁾/g Endo-1,4-beta-xylanase : 26000 U⁽⁶⁾/g	Chickens for fattening	-	Endo-1,4-beta- glucanase: 200 U⁽⁴⁾ Endo-1,3(4)- beta-glucanase : 450 U⁽⁵⁾ Endo-1,4-beta- xylanase : 650 U⁽⁶⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: endo-1,4-beta- glucanase: 800 to 1200 U⁽⁴⁾,endo-1,3(4)-beta- glucanase : 1800 to 2700 U⁽⁵⁾,endo-1,4-beta-xylanase : 2600 to 3900 U⁽⁶⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 20% barley and/or 25% rye.	30.6.2004 ^(I)(a)
			Laying hens	-	Endo-1,4-beta- glucanase: 640 U⁽³⁾ Endo-1,3(4)- beta-glucanase : 1440 U⁽⁵⁾ Endo-1,4-beta- xylanase : 2080 U⁽⁶⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: endo-1,4-beta- glucanase: 640 to 1280 U⁽⁴⁾,endo-1,3(4)-beta- glucanase : 1440 to 2880 U⁽⁵⁾,endo-1,4-beta-xylanase : 2080 to 4160 U⁽⁶⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 20% barley and/or 25% rye.	30.6.2004 ^(I)(a)
			Turkeys for fattening	-	Endo-1,4-beta- glucanase: 800 U⁽⁴⁾ Endo-1,3(4)- beta-glucanase : 1800 U⁽⁵⁾ Endo-1,4-beta- xylanase : 2600 U⁽⁶⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: endo-1,4-beta- glucanase: 800 to 1200 U⁽⁴⁾,endo-1,3(4)-beta- glucanase : 1800 to 2700 U⁽⁵⁾,endo-1,4-beta-xylanase : 2600 to 3900 U⁽⁶⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 20% barley.	30.6.2004 ^(I)(a)
13	Endo-1,3(4)-beta- glucanase EC 3.2.1.6 Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1,3(4)-beta- glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CBS 357.94) having a minimum activity of: Powder form: Endo-1,3(4)-beta- glucanase: 8000 BGU⁽⁷⁾/g Endo-1,4-beta-xylanase: 11000 EXU⁽⁸⁾/g Granulated form: Endo-1,3(4)-beta- glucanase: 6000 BGU⁽⁷⁾/g Endo-1,4-beta-xylanase: 8250 EXU⁽⁸⁾/g Liquid form: Endo-1,3(4)-beta- glucanase 2000 BGU⁽⁷⁾/ml Endo-1,4-beta- xylanase: 2750 EXU⁽⁸⁾/ml	Chickens for fattening	-	100 BGU⁽⁷⁾ 130 EXU⁽⁸⁾	^-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 100 BGU⁽⁷⁾, 130 EXU⁽⁸⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% wheat and 30% barley or 20% rye.	30.6.2004 ^(I)(a)
			Laying hens	-	600 BGU⁽⁷⁾ 800 EXU⁽⁸⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 600 BGU⁽⁷⁾, 800 EXU⁽⁸⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40% wheat and more than 30% barley.	30.6.2004 ^(I)(d)
			Turkeys for fattening	-	600 BGU⁽⁷⁾ 800 EXU⁽⁸⁾	-	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 600 BGU⁽⁷⁾, 800 EXU⁽⁸⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% wheat or more than 30% rye.	30.6.2004 ^(I)(d)
14	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 520.94) having minimum activities of: Solid form: 600 U⁽⁹⁾/g Liquid form: 300 U⁽⁹⁾/ml	Chickens for fattening	-	Endo-1,4-beta- xylanase: 300 U⁽⁹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 300 to 600 U⁽⁹⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50% wheat.	30.6.2004 ^(I)(a)
15	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo -1, 3(4)-beta- glucanase produced by Trichoderma viride (CBS 517.94) having a minimum activity of: Solid form: 650 U⁽¹⁰⁾/g Liquid form: 325 U⁽¹⁰⁾/ml	Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 325 U⁽¹⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 325 to 650 U⁽¹⁰⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50% barley.	30.6.2004 ^(I)(a)
16	Endo-1,4-beta- glucanase EC 3.2.1.4	Preparation of endo-1, 4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of: Liquid form: 2000 CU⁽¹¹⁾/ml	Chickens for fattening	-	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	30.6.2004 ^(I)(a)
			Laying hens	-	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	30.6.2004 ^(I)(a)
			Piglets	Four months	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	30.6.2004 ^(I)(a)
			Pigs for fattening	-	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	30.6.2004 ^(I)(a)
		Preparation of endo-1, 4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of: Solid form: 2000 CU⁽¹¹⁾/g Liquid form: 2000 CU⁽¹¹⁾/ml	Chickens for fattening	-	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	17.7.2004 ^(I)(c)
			Laying hens	-	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	17.7.2004 ^(I)(c)
			Piglets	Four months	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	17.7.2004 ^(I)(c)
			Pigs for fattening	-	250 CU⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 CU⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 40% barley.	17.7.2004 ^(I)(c)
17	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of: Liquid form: 2000 EPU⁽¹²⁾/ml	Chickens for fattening	-	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	30.6.2004 ^(I)(a)
			Laying hens	-	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	30.6.2004 ^(I)(a)
			Piglets	Four months	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	30.6.2004 ^(I)(a)
			Pigs for fattening	-	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	30.6.2004 ^(I)(a)
		Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of: Solid form: 6000 EPU⁽¹²⁾/g	Chickens for fattening	-	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	14.7.2004 ^(I)(c)
			Laying hens	-	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	14.7.2004 ^(I)(c)
			Piglets	Four months	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	14.7.2004 ^(I)(c)
			Pigs for fattening		750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 40% wheat or maize.	14.7.2004 ^(I)(c)
			Turkeys for fattening	-	750 EPU⁽¹²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 1500 to 3000 EPU⁽¹²⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly arabinoxylans) e.g. containing more than 35% wheat or maize.	14.7.2004 ^(I)(c)
18	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus niger (MUCL 39199) having a minimum activity of: Solid form: 2000 AGL⁽¹³⁾/g Liquid form: 500 AGL⁽¹³⁾/ml	Chickens for fattening	-	100 AGL⁽¹³⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 100 AGL⁽¹³⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glycans) e.g. containing more than 40% barley and 20% wheat.	30.6.2004 ^(I)(a)
19	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus niger (MUCL 39199) having a minimum activity of: Solid form: 1500 AGL⁽¹⁴⁾/g Liquid form: 200 AGL⁽¹⁴⁾/g	Chickens for fattening	-	25 AGL⁽¹⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 25 to 100 AGL⁽¹⁴⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 50% barley.	30.6.2004 ^(I)(a)
20	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (MUCL 39203) having a minimum activity of: Solid form: 2000 AXC⁽¹⁵⁾/g Liquid form: 500 AXC⁽¹⁵⁾/ml	Chicken for fattening	-	100 AXC⁽¹⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 100 AXC⁽¹⁵⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 40% wheat or rye.	30.6.2004 ^(I)(a)
21	Endo-1,4-beta- xylanase EC 3.2.1.8.	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (MUCL 39203) having a minimum activity of: Solid form: 1500 AXC⁽¹⁶⁾/g Liquid form: 200 AXC⁽¹⁶⁾/g	Chickens for fattening	-	25 AXC⁽¹⁶⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 25 to 100 AXC⁽¹⁶⁾. 3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 50% wheat.	30.6.2004 ^(II)(a)