70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (3)

N°
Additive Chemical name, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

22

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Preparation of endo -1,3(4)-beta- glucanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum activity of:

Solid form: 70000 BGN⁽¹⁾/g
Liquid form: 14000 BGN⁽¹⁾/ml

Chickens for fattening

-

1050 BGN⁽¹⁾

^-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff: 2800 BGN⁽¹⁾.

3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50% barley.

30.6.2004 ^(I)(a)

23

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo -1,4-beta-xylanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum activity of:

Solid form: 70000 IFP⁽²⁾/g
Liquid form: 7000 IFP⁽²⁾/ml

Chickens for fattening

-

1050 IFP⁽²⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff: 1400 IFP⁽²⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 56% wheat.

30.6.2004 ^(I)(a)

Turkeys for fattening

-

700 IFP⁽²⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff: 1400 IFP⁽²⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40% wheat.

28.2.2005 ^(I)(e)

Laying hens

-

840 IFP⁽²⁾

-

1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of feedingstuff: 840 IFP⁽²⁾

3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40% wheat.

28.2.2005 ^(I)(e)

24

Endo-1,4-beta-
xylanase EC 3.2.1.8

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Preparation of endo -1,4-beta-xylanase and endo -1,3(4)-beta-glucanase produced by Aspergillus niger (CNCM I-1517) having a minimum activity of:

28000 QXU⁽³⁾/g
140000 QGU⁽⁴⁾/g

Chickens for fattening

-

420 QXU⁽³⁾

2100 QGU⁽⁴⁾

1120 QXU⁽³⁾

5600 QGU⁽⁴⁾

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended dose per kg of complete feedingstuff: 560 QXU⁽³⁾,
2800 QGU⁽⁴⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% wheat and 30% barley.

30.6.2004 ^(I)(a)

25

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo -1,3(4)-beta-glucanase and endo -1,4-beta-xylanase produced by Aspergillus niger (NRRL 25541) having a minimum activity of:

Endo-1,3(4)-beta-
glucanase: 1100 U⁽⁵⁾/g

Endo-1,4-beta-xylanase: 1600 U⁽⁶⁾/g

Chickens for fattening

-

Endo-1,3(4)-
beta-glucanase: 138 U⁽⁵⁾

Endo-1,4-beta-
xylanase: 200 U⁽⁶⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 138 U⁽⁵⁾,
endo-1,4-beta-xylanase: 200 U⁽⁶⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50% barley or 30% wheat and 30% maize.

30.6.2004 ^(I)(a)

Laying hens

-

Endo-1,3(4)-
beta-glucanase: 138 U⁽⁵⁾

Endo-1,4-beta-
xylanase: 200 U⁽⁶⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 138 U⁽⁵⁾,
endo-1,4-beta-xylanase: 200 U⁽⁶⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50% barley or 30% wheat and 30% maize.

30.6.2004 ^(I)(a)

26

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Preparation of endo-1,3(4)-beta-
glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:

Solid form: 350000 BU⁽⁷⁾/g
Liquid form: 50000 BU⁽⁷⁾/g

Chickens for fattening

-

23000 BU⁽⁷⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 23000 to 50000 BU⁽⁷⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 20% barley or 30% rye.

30.6.2004 ^(I)(b)

Piglets

Four months

26000 BU⁽⁷⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 26000 to 35000 BU⁽⁷⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 60% barley or wheat.

30.6.2004 ^(I)(b)

27

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6

Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 529.94) and endo-
1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having minimum activities of:

Solid form: 200000 BXU⁽⁸⁾/g
200000 BU⁽⁹⁾/g

Liquid form: 30000 BXU⁽⁸⁾/g
30000 BU⁽⁹⁾/g

Chickens for fattening

-

2500 BXU⁽⁸⁾

2500 BU⁽⁹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

10000 BXU⁽⁸⁾,
10000 BU⁽⁹⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and glucans), e.g. containing more than 40% wheat or 30% rye.

30.6.2004 ^(I)(b)

Piglets

2 months

7500 BXU⁽⁸⁾

7500 BU⁽⁹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

7500 to 15000 BXU⁽⁸⁾,
7500 to 15000 BU⁽⁹⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50% wheat.

28.2.2005 ^(I)(e)

28

3-Phytase EC 3.1.3.8

Preparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum activity of:

Solid form: 5000 PPU⁽¹⁰⁾/g
Liquid form: 1000 PPU⁽¹⁰⁾/g

Piglets

Four months

250 PPU⁽¹⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 750 PPU⁽¹⁰⁾.

3.For use in compound feed rich in phytates e.g. containing more than 50% cereals (corn, barley, wheat), tapioca, oilseeds and pulses.

30.6.2004 ^(I)(b)

Pigs for fattening

-

500 PPU⁽¹⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 750 PPU⁽¹⁰⁾.

3.For use in compound feed rich in phytates e.g. containing more than 50% cereals (corn, barley, wheat), tapioca, oilseeds and pulses.

30.6.2004 ^(I)(b)

Chicken for fattening

-

500 PPU

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 500 to 750 PPU⁽¹⁰⁾.

3.For use in compound feed containing more than 0,22% phytin bound phosphorus.

28.2.2005 ^(I)(e)

29

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Preparation of endo-1,
3(4)-beta-glucanase produced by Geosmithia emersonii (IMI SD 133) having a minimum activity of:

5500 U⁽¹¹⁾/g

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 250 U⁽¹¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 250 U⁽¹¹⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans) e.g. containing more than 50% barley.

30.6.2004 ^(I)(c)

30

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo-1,
3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Penicillium funiculosum (IMI SD 101) having a minimum activity of:

Powder form:
Endo-1,3(4)-beta-
glucanase: 2000 U⁽¹²⁾/g
Endo-1,4-beta-xylanase: 1400 U⁽¹³⁾/g

Liquid form:
Endo-1,3(4)-beta-
glucanase: 500 U⁽¹²⁾/ml
Endo-1,4-beta-xylanase: 350 U⁽¹³⁾/ml

Chickens for fattening

-

Endo-1,3(4)-
beta-glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-
xylanase: 70 U⁽¹³⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,3(4)-beta-
glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-xylanase: 70 U⁽¹³⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 50% barley or 60% wheat.

30.6.2004 ^(I)(c)

Turkeys for fattening

-

Endo-1,3(4)-
beta-glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-
xylanase: 70 U⁽¹³⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,3(4)-beta-
glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-xylanase: 70 U⁽¹³⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 50% wheat.

28.2.2005 ^(I)(e)

Laying hens

-

Endo-1,3(4)-
beta-glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-
xylanase: 70 U⁽¹³⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,3(4)-beta-
glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-xylanase: 70 U⁽¹³⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 60% barley or 30% wheat.

28.2.2005 ^(I)(e)

Pigs for fattening

-

Endo-1,3(4)-
beta-glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-
xylanase: 70 U⁽¹³⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,3(4)-beta-
glucanase: 100 U⁽¹²⁾

Endo-1,4-beta-xylanase: 70 U⁽¹³⁾.

3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 50% barley or 60% wheat.

28.2.2005 ^(I)(e)

31

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CBS 614-94) having a minimum activity of:

Solid form: 300 EU⁽¹⁴⁾/g
Liquid form:1000 EU⁽¹⁴⁾/g

Chickens for fattening

-

600 EU⁽¹⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 600 EU⁽¹⁴⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 60% wheat.

30.6.2004 ^(I)(c)

Laying hens

-

300 EU⁽¹⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff: 600 EU⁽¹⁴⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 60% wheat.

30.6.2004 ^(I)(c)

32

Endo-1,3(4)-beta-
glucanase EC 3.2.1.6

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of:

Endo-1,3(4)-beta-
glucanase: 200 U⁽¹⁵⁾/ml

Chicken for fattening

-

Endo-1,3(4)-beta-
glucanase: 100 U⁽¹⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 100 U⁽¹⁵⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 30% barley.

30.6.2004 ^(I)(d)

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of:

Endo-1,3(4)-beta-
glucanase: 1200 U⁽¹⁵⁾/ml

Piglets

Four months

Endo-1,3(4)-beta-
glucanase: 400 U⁽¹⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,3(4)-beta-
glucanase: 400 U⁽¹⁵⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 55% barley.

30.6.2004 ^(I)(d)

Pigs for fattening

-

Endo-1,3(4)-beta-
glucanase: 500 U⁽¹⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,3(4)-beta-
glucanase: 500 U⁽¹⁵⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 70% barley.

30.6.2004 ^(I)(d)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)

(a) First authorisation Commission Regulation (EC) N° 1436/98 (OJ L 191, 7.7.1998)
(b) First authorisation Commission Regulation (EC) N° 2374/98 (OJ L 295, 4.11.1998)
(c) First authorisation Commission Regulation (EC) N° 866/1999 (OJ L 108, 27.4.1999)
(d) First authorisation Commission Regulation (EC) N° 1411/1999 (OJ L 164, 30.6.1999)
(e) First authorisation Commission Regulation (EC) N° 418/2001 (OJ L 62, 2.3.2001)

(1) 1 BGN is the amount of enzyme which liberates 1 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50°C.
(2) 1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50°C.
(3) 1 QXU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 5,1 and 50°C.
(4) 1 QGU is the amount of enzyme which liberates 1 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50°C.
(5) 1 U is the amount of enzyme which liberates 1 micromoles of reducing sugars (glucose equivalents) from oat beta-glucan per minute at pH 4,0 and 30°C.
(6) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,0 and 30°C.
(7) 1 BU is the amount of enzyme which liberates 0.06 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50°C.
(8) 1 BXU is the amount of enzyme which liberates 0.06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50°C.
(9) 1 BU is the amount of enzyme which liberates 0.06 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50°C.
(10) 1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate fom sodium phytate per minute at pH 5,0 and 37°C.
(11) 1 U is the amount of enzyme which liberates 2,78 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50°C.
(12) 1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50°C.
(13) 1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50°C.
(14) 1 EU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,5 and 40°C.
(15) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.

[back to Directive text]

N°	Additive	Chemical name, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

22	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo -1,3(4)-beta- glucanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum activity of: Solid form: 70000 BGN⁽¹⁾/g Liquid form: 14000 BGN⁽¹⁾/ml	Chickens for fattening	-	1050 BGN⁽¹⁾	^-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 2800 BGN⁽¹⁾. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50% barley.	30.6.2004 ^(I)(a)
23	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1,4-beta-xylanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum activity of: Solid form: 70000 IFP⁽²⁾/g Liquid form: 7000 IFP⁽²⁾/ml	Chickens for fattening	-	1050 IFP⁽²⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 1400 IFP⁽²⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 56% wheat.	30.6.2004 ^(I)(a)
			Turkeys for fattening	-	700 IFP⁽²⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 1400 IFP⁽²⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40% wheat.	28.2.2005 ^(I)(e)
			Laying hens	-	840 IFP⁽²⁾	-	1. In the conditions of use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of feedingstuff: 840 IFP⁽²⁾ 3. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40% wheat.	28.2.2005 ^(I)(e)
24	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo -1,4-beta-xylanase and endo -1,3(4)-beta-glucanase produced by Aspergillus niger (CNCM I-1517) having a minimum activity of: 28000 QXU⁽³⁾/g 140000 QGU⁽⁴⁾/g	Chickens for fattening	-	420 QXU⁽³⁾ 2100 QGU⁽⁴⁾	1120 QXU⁽³⁾ 5600 QGU⁽⁴⁾	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: 560 QXU⁽³⁾, 2800 QGU⁽⁴⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 30% wheat and 30% barley.	30.6.2004 ^(I)(a)
25	Endo-1,3(4)-beta- glucanase EC 3.2.1.6 Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo -1,3(4)-beta-glucanase and endo -1,4-beta-xylanase produced by Aspergillus niger (NRRL 25541) having a minimum activity of: Endo-1,3(4)-beta- glucanase: 1100 U⁽⁵⁾/g Endo-1,4-beta-xylanase: 1600 U⁽⁶⁾/g	Chickens for fattening	-	Endo-1,3(4)- beta-glucanase: 138 U⁽⁵⁾ Endo-1,4-beta- xylanase: 200 U⁽⁶⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 138 U⁽⁵⁾, endo-1,4-beta-xylanase: 200 U⁽⁶⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50% barley or 30% wheat and 30% maize.	30.6.2004 ^(I)(a)
25			Laying hens	-	Endo-1,3(4)- beta-glucanase: 138 U⁽⁵⁾ Endo-1,4-beta- xylanase: 200 U⁽⁶⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 138 U⁽⁵⁾, endo-1,4-beta-xylanase: 200 U⁽⁶⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50% barley or 30% wheat and 30% maize.	30.6.2004 ^(I)(a)
26	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1,3(4)-beta- glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of: Solid form: 350000 BU⁽⁷⁾/g Liquid form: 50000 BU⁽⁷⁾/g	Chickens for fattening	-	23000 BU⁽⁷⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 23000 to 50000 BU⁽⁷⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 20% barley or 30% rye.	30.6.2004 ^(I)(b)
26	Endo-1,3(4)-beta- glucanase EC 3.2.1.6		Piglets	Four months	26000 BU⁽⁷⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 26000 to 35000 BU⁽⁷⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 60% barley or wheat.	30.6.2004 ^(I)(b)
27	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 529.94) and endo- 1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having minimum activities of: Solid form: 200000 BXU⁽⁸⁾/g 200000 BU⁽⁹⁾/g Liquid form: 30000 BXU⁽⁸⁾/g 30000 BU⁽⁹⁾/g	Chickens for fattening	-	2500 BXU⁽⁸⁾ 2500 BU⁽⁹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 10000 BXU⁽⁸⁾, 10000 BU⁽⁹⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and glucans), e.g. containing more than 40% wheat or 30% rye.	30.6.2004 ^(I)(b)
27			Piglets	2 months	7500 BXU⁽⁸⁾ 7500 BU⁽⁹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 7500 to 15000 BXU⁽⁸⁾, 7500 to 15000 BU⁽⁹⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50% wheat.	28.2.2005 ^(I)(e)
28	3-Phytase EC 3.1.3.8	Preparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum activity of: Solid form: 5000 PPU⁽¹⁰⁾/g Liquid form: 1000 PPU⁽¹⁰⁾/g	Piglets	Four months	250 PPU⁽¹⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 750 PPU⁽¹⁰⁾. 3.For use in compound feed rich in phytates e.g. containing more than 50% cereals (corn, barley, wheat), tapioca, oilseeds and pulses.	30.6.2004 ^(I)(b)
			Pigs for fattening	-	500 PPU⁽¹⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 750 PPU⁽¹⁰⁾. 3.For use in compound feed rich in phytates e.g. containing more than 50% cereals (corn, barley, wheat), tapioca, oilseeds and pulses.	30.6.2004 ^(I)(b)
			Chicken for fattening	-	500 PPU	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 750 PPU⁽¹⁰⁾. 3.For use in compound feed containing more than 0,22% phytin bound phosphorus.	28.2.2005 ^(I)(e)
29	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1, 3(4)-beta-glucanase produced by Geosmithia emersonii (IMI SD 133) having a minimum activity of: 5500 U⁽¹¹⁾/g	Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 250 U⁽¹¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 250 U⁽¹¹⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans) e.g. containing more than 50% barley.	30.6.2004 ^(I)(c)
30	Endo-1,3(4)-beta- glucanase EC 3.2.1.6 Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1, 3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Penicillium funiculosum (IMI SD 101) having a minimum activity of: Powder form: Endo-1,3(4)-beta- glucanase: 2000 U⁽¹²⁾/g Endo-1,4-beta-xylanase: 1400 U⁽¹³⁾/g Liquid form: Endo-1,3(4)-beta- glucanase: 500 U⁽¹²⁾/ml Endo-1,4-beta-xylanase: 350 U⁽¹³⁾/ml	Chickens for fattening	-	Endo-1,3(4)- beta-glucanase: 100 U⁽¹²⁾ Endo-1,4-beta- xylanase: 70 U⁽¹³⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,3(4)-beta- glucanase: 100 U⁽¹²⁾ Endo-1,4-beta-xylanase: 70 U⁽¹³⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 50% barley or 60% wheat.	30.6.2004 ^(I)(c)
			Turkeys for fattening	-	Endo-1,3(4)- beta-glucanase: 100 U⁽¹²⁾ Endo-1,4-beta- xylanase: 70 U⁽¹³⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,3(4)-beta- glucanase: 100 U⁽¹²⁾ Endo-1,4-beta-xylanase: 70 U⁽¹³⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 50% wheat.	28.2.2005 ^(I)(e)
			Laying hens	-	Endo-1,3(4)- beta-glucanase: 100 U⁽¹²⁾ Endo-1,4-beta- xylanase: 70 U⁽¹³⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,3(4)-beta- glucanase: 100 U⁽¹²⁾ Endo-1,4-beta-xylanase: 70 U⁽¹³⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 60% barley or 30% wheat.	28.2.2005 ^(I)(e)
			Pigs for fattening	-	Endo-1,3(4)- beta-glucanase: 100 U⁽¹²⁾ Endo-1,4-beta- xylanase: 70 U⁽¹³⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,3(4)-beta- glucanase: 100 U⁽¹²⁾ Endo-1,4-beta-xylanase: 70 U⁽¹³⁾. 3.For use in compound feed rich in non-starch polysaccharides,, (mainly beta-glucans and arabinoxylans) e.g. containing more than 50% barley or 60% wheat.	28.2.2005 ^(I)(e)
31	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CBS 614-94) having a minimum activity of: Solid form: 300 EU⁽¹⁴⁾/g Liquid form:1000 EU⁽¹⁴⁾/g	Chickens for fattening	-	600 EU⁽¹⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 600 EU⁽¹⁴⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 60% wheat.	30.6.2004 ^(I)(c)
31	Endo-1,4-beta- xylanase EC 3.2.1.8		Laying hens	-	300 EU⁽¹⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 600 EU⁽¹⁴⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 60% wheat.	30.6.2004 ^(I)(c)
32	Endo-1,3(4)-beta- glucanase EC 3.2.1.6	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of: Endo-1,3(4)-beta- glucanase: 200 U⁽¹⁵⁾/ml	Chicken for fattening	-	Endo-1,3(4)-beta- glucanase: 100 U⁽¹⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 100 U⁽¹⁵⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 30% barley.	30.6.2004 ^(I)(d)
		Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of: Endo-1,3(4)-beta- glucanase: 1200 U⁽¹⁵⁾/ml	Piglets	Four months	Endo-1,3(4)-beta- glucanase: 400 U⁽¹⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,3(4)-beta- glucanase: 400 U⁽¹⁵⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 55% barley.	30.6.2004 ^(I)(d)
			Pigs for fattening	-	Endo-1,3(4)-beta- glucanase: 500 U⁽¹⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,3(4)-beta- glucanase: 500 U⁽¹⁵⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly beta-glucans) e.g. containing more than 70% barley.	30.6.2004 ^(I)(d)