70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (4)

N°
Additive Chemical name, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

33

Endo-1,4-beta-
xylanase EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:

Powder form:
Endo-1,4-beta-
xylanase: 2000 U⁽¹⁾/g

Liquid form:
Endo-1,4-beta-
xylanase: 5000 U⁽¹⁾/ml

Chicken for fattening

-

Endo-1,4-beta-
xylanase: 500 U⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase: 500 to 2500 U⁽¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 55% wheat or 60% rye.

30.6.2004 ^(I)(a)

Laying hens

-

Endo-1,4-beta-
xylanase: 2000 U⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase: 2000 U⁽¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 35% wheat.

30.6.2004 ^(I)(a)

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:

Powder form:
Endo-1,4-beta-
xylanase: 4000 U⁽¹⁾/g

Liquid form:
Endo-1,4-beta-
xylanase: 10000 U⁽¹⁾/ml

Piglets

Four months

Endo-1,4-beta-
xylanase: 5000 U⁽⁴⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase: 5000 U⁽¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 45% wheat.

30.6.2004 ^(I)(a)

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:

Powder form:
Endo-1,4-beta-
xylanase: 4000 U⁽¹⁾/g

Liquid form:
Endo-1,4-beta-
xylanase: 8000 U⁽¹⁾/ml

Pigs for fattening

-

Endo-1,4-beta-
xylanase: 4000 U⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase: 4000 U⁽¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 35% wheat.

30.6.2004 ^(I)(a)

34

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Alpha-amylase
EC 3.2.1.1.

Preparation of endo-1,
3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Aspergillus niger (NRRL 25541) and alpha-amylase produced by Aspergillus oryzae (ATCC 66 222) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 275 U⁽²⁾/g
endo-1,4-beta-xylanase: 400 U⁽³⁾/g
alpha-amylase: 3100 U⁽⁴⁾/g

Piglets

Four months

Endo-1,3(4)-
beta-glucanase: 165 U⁽²⁾

Endo-1,4-beta-
xylanase: 240 U⁽³⁾

Alpha-amylase: 1860 U⁽⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 165 U⁽²⁾,
endo-1,4-beta-xylanase: 240 U⁽³⁾,
alpha-amylase: 1860 U⁽⁴⁾.

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 45% barley and 10% wheat or 10% maize.

26.7.2004 ^(I)(b)

35

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 80 U⁽⁵⁾/g
endo-1,4-beta-xylanase: 180 U⁽⁶⁾/g

Laying hens

-

Endo-1,3(4)-
beta-glucanase: 80 U⁽⁵⁾

Endo-1,4-beta-
xylanase: 180 U⁽⁶⁾

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 80 U⁽⁵⁾,
endo-1,4-beta-xylanase: 180 U⁽⁶⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.

26.7.2004 ^(I)(b)

36

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 300 U⁽⁷⁾/g
endo-1,4-beta-xylanase: 300 U⁽⁸⁾/g

Chicken for fattening

-

Endo-1,3(4)-
beta-glucanase: 300 U⁽⁷⁾

Endo-1,4-beta-
xylanase: 300 U⁽⁸⁾

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 300 U⁽⁷⁾,
endo-1,4-beta-xylanase: 300 U⁽⁸⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40% barley.

26.7.2004 ^(I)(b)

Laying hens

Endo-1,3(4)-
beta-glucanase: 300 U⁽⁷⁾

Endo-1,4-beta-
xylanase: 300 U⁽⁸⁾

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 300 U⁽⁷⁾,
endo-1,4-beta-xylanase: 300 U⁽⁸⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 35% barley.

26.7.2004 ^(I)(b)

37

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Subtilisin
EC 3.4.21.62

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) with a minimum activity of:

endo-1,4-beta-xylanase: 2500 U⁽⁹⁾/g
subtilisin: 800 U⁽¹⁰⁾/g

Chicken for fattening

-

Endo-1,4-beta-
xylanase: 500 U⁽⁹⁾Subtilisin: 160 U⁽¹⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-xylanase: 500 to 2500 U⁽⁹⁾,
subtilisin: 160-800 U⁽¹⁰⁾.

3.For use in compound feed e.g. containing more than 65% wheat.

26.7.2004 ^(I)(b)

Turkey

-

Endo-1,4-beta-
xylanase: 825 U⁽⁹⁾

Subtilisin: 265 U⁽¹⁰⁾

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-xylanase: 825 to 2500 U⁽⁹⁾,
subtilisin: 265-800 U⁽¹⁰⁾.

3.For use in compound feed e.g. containing more than 45% wheat.

26.7.2004 ^(I)(b)

38

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Subtilisin
EC 3.4.21.62

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:

endo-1,4-beta-xylanase: 5000 U⁽¹¹⁾/g
subtilisin: 500 U⁽¹²⁾/g

Piglets

Four months

Endo-1,4-beta-
xylanase: 5000 U⁽¹¹⁾

Subtilisin: 500 U⁽¹²⁾

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-xylanase: 5000 U⁽¹¹⁾,
subtilisin: 500 U⁽¹²⁾.

3.For use in compound feed e.g. containing more than 40% wheat.

26.7.2004 ^(I)(b)

39

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 400 U⁽¹³⁾/g
endo-1,4-beta-xylanase: 400 U⁽¹⁴⁾/g

Pigs for fattening

-

Endo-1,3(4)-
beta-glucanase: 400 U⁽¹³⁾

Endo-1,4-beta-
xylanase: 400 U⁽¹⁴⁾

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 400 U⁽¹³⁾,
endo-1,4-beta-xylanase: 400 U⁽¹⁴⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 65% barley.

26.7.2004 ^(I)(b)

40

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Subtilisin
EC 3.4.21.62

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 1000 U⁽¹⁵⁾/g
endo-1,4-beta-xylanase: 300 U⁽¹⁶⁾/g
subtilisin: 800 U⁽¹⁷⁾/g

Chickens for fattening

-

Endo-1,3(4)-
beta-glucanase: 30 U⁽¹⁵⁾

Endo-1,4-beta-
xylanase: 90 U⁽¹⁶⁾

Subtilisin: 240 U⁽¹⁷⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 30 to 100 U⁽¹⁵⁾,
endo-1,4-beta-xylanase: 90 to 300 U⁽¹⁶⁾,
subtilisin: 240 to 800 U⁽¹⁷⁾.

3.For use in compound feed e.g. containing more than 60% barley.

26.7.2004 ^(I)(b)

41

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Subtilisin
EC 3.4.21.62

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 100 U⁽¹⁸⁾/g
endo-1,4-beta-xylanase: 2500 U⁽¹⁹⁾/g
subtilisin: 800 U⁽²⁰⁾/g

Chickens for fattening

-

Endo-1,3(4)-
beta-glucanase: 25 U⁽¹⁸⁾

Endo-1,4-beta-
xylanase: 625 U⁽¹⁹⁾

Subtilisin: 200 U⁽²⁰⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 25 to 100 U⁽¹⁸⁾,
endo-1,4-beta-xylanase: 625 to 2500 U⁽¹⁹⁾,
subtilisin: 200 to 800 U⁽²⁰⁾.

3.For use in compound feed e.g. containing more than 30% wheat and 10% barley.

26.7.2004 ^(I)(b)

Laying hens

-

Endo-1,3(4)-
beta-glucanase: 100 U⁽¹⁸⁾

Endo-1,4-beta-
xylanase: 2500 U⁽¹⁹⁾

Subtilisin: 800 U⁽²⁰⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 100 U⁽¹⁸⁾,
endo-1,4-beta-xylanase: 2500 U⁽¹⁹⁾,
subtilisin: 800 U⁽²⁰⁾.

3.For use in compound feed e.g. containing more than 50% wheat and 25% barley.

26.7.2004 ^(I)(b)

42

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Preparation of endo-1,
4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:

solid form: 4000 U⁽²¹⁾/g

Characteristics of authorised preparation:

endo-1,4-beta-xylanase: 1,99%,
wheat: 97,7%,
calcium propionate: 0,3%,
lecithin: 0,01%.

Piglets

Four months

Endo-1,4-beta-
xylanase: 4000 U⁽²¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase: 4000 U⁽²¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans), e.g. containing more than 60% wheat.

26.7.2004 ^(I)(b)

Pigs for fattening

-

Endo-1,4-beta-
xylanase: 4000 U⁽²¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase: 4000 U⁽²¹⁾.

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans), e.g. containing more than 60% wheat.

17.7.2001 ^(I)(c)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)

(a) First authorisation Commission Regulation (EC) N° 1411/1999 (OJ L 164, 30.6.1999)
(b) First authorisation Commission Regulation (EC) N° 1636/1999 (OJ L 194, 27.7.1999)
(c) First authorisation Commission Regulation (EC) N° 1353/2000 (OJ L 155, 28.6.2000)

(1) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(2) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from oat beta-glucan per minute at pH 4,0 and 30°C.
(3) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from oat xylan per minute at pH 4,0 and 30°C.
(4) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from wheat starch per minute at pH 4,0 and 30°C.
(5) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(6) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(7) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(8) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(9) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(10) 1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40°C.
(11) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(12) 1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40°C.
(13) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(14) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(15) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(16) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(17) 1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40°C.
(18) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(19) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(20) 1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40°C.
(21) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.

[back to Directive text]

N°	Additive	Chemical name, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

33	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of: Powder form: Endo-1,4-beta- xylanase: 2000 U⁽¹⁾/g Liquid form: Endo-1,4-beta- xylanase: 5000 U⁽¹⁾/ml	Chicken for fattening	-	Endo-1,4-beta- xylanase: 500 U⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 500 to 2500 U⁽¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 55% wheat or 60% rye.	30.6.2004 ^(I)(a)
			Laying hens	-	Endo-1,4-beta- xylanase: 2000 U⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 2000 U⁽¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 35% wheat.	30.6.2004 ^(I)(a)
		Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of: Powder form: Endo-1,4-beta- xylanase: 4000 U⁽¹⁾/g Liquid form: Endo-1,4-beta- xylanase: 10000 U⁽¹⁾/ml	Piglets	Four months	Endo-1,4-beta- xylanase: 5000 U⁽⁴⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 5000 U⁽¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 45% wheat.	30.6.2004 ^(I)(a)
		Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of: Powder form: Endo-1,4-beta- xylanase: 4000 U⁽¹⁾/g Liquid form: Endo-1,4-beta- xylanase: 8000 U⁽¹⁾/ml	Pigs for fattening	-	Endo-1,4-beta- xylanase: 4000 U⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 4000 U⁽¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 35% wheat.	30.6.2004 ^(I)(a)
34	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Alpha-amylase EC 3.2.1.1.	Preparation of endo-1, 3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Aspergillus niger (NRRL 25541) and alpha-amylase produced by Aspergillus oryzae (ATCC 66 222) having a minimum activity of: endo-1,3(4)-beta- glucanase: 275 U⁽²⁾/g endo-1,4-beta-xylanase: 400 U⁽³⁾/g alpha-amylase: 3100 U⁽⁴⁾/g	Piglets	Four months	Endo-1,3(4)- beta-glucanase: 165 U⁽²⁾ Endo-1,4-beta- xylanase: 240 U⁽³⁾ Alpha-amylase: 1860 U⁽⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 165 U⁽²⁾, endo-1,4-beta-xylanase: 240 U⁽³⁾, alpha-amylase: 1860 U⁽⁴⁾. 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 45% barley and 10% wheat or 10% maize.	26.7.2004 ^(I)(b)
35	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8.	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of: endo-1,3(4)-beta- glucanase: 80 U⁽⁵⁾/g endo-1,4-beta-xylanase: 180 U⁽⁶⁾/g	Laying hens	-	Endo-1,3(4)- beta-glucanase: 80 U⁽⁵⁾ Endo-1,4-beta- xylanase: 180 U⁽⁶⁾	- -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 80 U⁽⁵⁾, endo-1,4-beta-xylanase: 180 U⁽⁶⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 60% barley.	26.7.2004 ^(I)(b)
36	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8.	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of: endo-1,3(4)-beta- glucanase: 300 U⁽⁷⁾/g endo-1,4-beta-xylanase: 300 U⁽⁸⁾/g	Chicken for fattening	-	Endo-1,3(4)- beta-glucanase: 300 U⁽⁷⁾ Endo-1,4-beta- xylanase: 300 U⁽⁸⁾	- -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 300 U⁽⁷⁾, endo-1,4-beta-xylanase: 300 U⁽⁸⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40% barley.	26.7.2004 ^(I)(b)
36			Laying hens		Endo-1,3(4)- beta-glucanase: 300 U⁽⁷⁾ Endo-1,4-beta- xylanase: 300 U⁽⁸⁾	- -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 300 U⁽⁷⁾, endo-1,4-beta-xylanase: 300 U⁽⁸⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 35% barley.	26.7.2004 ^(I)(b)
37	Endo-1,4-beta- xylanase EC 3.2.1.8. Subtilisin EC 3.4.21.62	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) with a minimum activity of: endo-1,4-beta-xylanase: 2500 U⁽⁹⁾/g subtilisin: 800 U⁽¹⁰⁾/g	Chicken for fattening	-	Endo-1,4-beta- xylanase: 500 U⁽⁹⁾Subtilisin: 160 U⁽¹⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,4-beta-xylanase: 500 to 2500 U⁽⁹⁾, subtilisin: 160-800 U⁽¹⁰⁾. 3.For use in compound feed e.g. containing more than 65% wheat.	26.7.2004 ^(I)(b)
37			Turkey	-	Endo-1,4-beta- xylanase: 825 U⁽⁹⁾ Subtilisin: 265 U⁽¹⁰⁾	- -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,4-beta-xylanase: 825 to 2500 U⁽⁹⁾, subtilisin: 265-800 U⁽¹⁰⁾. 3.For use in compound feed e.g. containing more than 45% wheat.	26.7.2004 ^(I)(b)
38	Endo-1,4-beta- xylanase EC 3.2.1.8. Subtilisin EC 3.4.21.62	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of: endo-1,4-beta-xylanase: 5000 U⁽¹¹⁾/g subtilisin: 500 U⁽¹²⁾/g	Piglets	Four months	Endo-1,4-beta- xylanase: 5000 U⁽¹¹⁾ Subtilisin: 500 U⁽¹²⁾	- -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,4-beta-xylanase: 5000 U⁽¹¹⁾, subtilisin: 500 U⁽¹²⁾. 3.For use in compound feed e.g. containing more than 40% wheat.	26.7.2004 ^(I)(b)
39	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8.	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of: endo-1,3(4)-beta- glucanase: 400 U⁽¹³⁾/g endo-1,4-beta-xylanase: 400 U⁽¹⁴⁾/g	Pigs for fattening	-	Endo-1,3(4)- beta-glucanase: 400 U⁽¹³⁾ Endo-1,4-beta- xylanase: 400 U⁽¹⁴⁾	- -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 400 U⁽¹³⁾, endo-1,4-beta-xylanase: 400 U⁽¹⁴⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 65% barley.	26.7.2004 ^(I)(b)
40	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Subtilisin EC 3.4.21.62	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of: endo-1,3(4)-beta- glucanase: 1000 U⁽¹⁵⁾/g endo-1,4-beta-xylanase: 300 U⁽¹⁶⁾/g subtilisin: 800 U⁽¹⁷⁾/g	Chickens for fattening	-	Endo-1,3(4)- beta-glucanase: 30 U⁽¹⁵⁾ Endo-1,4-beta- xylanase: 90 U⁽¹⁶⁾ Subtilisin: 240 U⁽¹⁷⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 30 to 100 U⁽¹⁵⁾, endo-1,4-beta-xylanase: 90 to 300 U⁽¹⁶⁾, subtilisin: 240 to 800 U⁽¹⁷⁾. 3.For use in compound feed e.g. containing more than 60% barley.	26.7.2004 ^(I)(b)
41	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Subtilisin EC 3.4.21.62	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of: endo-1,3(4)-beta- glucanase: 100 U⁽¹⁸⁾/g endo-1,4-beta-xylanase: 2500 U⁽¹⁹⁾/g subtilisin: 800 U⁽²⁰⁾/g	Chickens for fattening	-	Endo-1,3(4)- beta-glucanase: 25 U⁽¹⁸⁾ Endo-1,4-beta- xylanase: 625 U⁽¹⁹⁾ Subtilisin: 200 U⁽²⁰⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 25 to 100 U⁽¹⁸⁾, endo-1,4-beta-xylanase: 625 to 2500 U⁽¹⁹⁾, subtilisin: 200 to 800 U⁽²⁰⁾. 3.For use in compound feed e.g. containing more than 30% wheat and 10% barley.	26.7.2004 ^(I)(b)
41			Laying hens	-	Endo-1,3(4)- beta-glucanase: 100 U⁽¹⁸⁾ Endo-1,4-beta- xylanase: 2500 U⁽¹⁹⁾ Subtilisin: 800 U⁽²⁰⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 100 U⁽¹⁸⁾, endo-1,4-beta-xylanase: 2500 U⁽¹⁹⁾, subtilisin: 800 U⁽²⁰⁾. 3.For use in compound feed e.g. containing more than 50% wheat and 25% barley.	26.7.2004 ^(I)(b)
42	Endo-1,4-beta- xylanase EC 3.2.1.8.	Preparation of endo-1, 4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of: solid form: 4000 U⁽²¹⁾/g Characteristics of authorised preparation: endo-1,4-beta-xylanase: 1,99%, wheat: 97,7%, calcium propionate: 0,3%, lecithin: 0,01%.	Piglets	Four months	Endo-1,4-beta- xylanase: 4000 U⁽²¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 4000 U⁽²¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans), e.g. containing more than 60% wheat.	26.7.2004 ^(I)(b)
42	Endo-1,4-beta- xylanase EC 3.2.1.8.		Pigs for fattening	-	Endo-1,4-beta- xylanase: 4000 U⁽²¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 4000 U⁽²¹⁾. 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans), e.g. containing more than 60% wheat.	17.7.2001 ^(I)(c)