70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (5)

N°
Additive Chemical name, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

43

Endo-1,4-beta-
xylanase EC 3.2.1.8

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Alpha-amylase
EC 3.2.1.1.

Preparation of endo-1,4-
beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135), endo-1,3(4)-beta-
glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of:

endo-1,4-beta-xylanase: 3975 U/g⁽²⁾

endo-1,3(4)-beta-
glucanase: 125 U/g⁽³⁾

alpha-amylase: 1000 U/g⁽⁴⁾

Piglets

4 months

Endo-1,4-beta-
xylanase: 3975 U/g⁽²⁾

Endo-1,3(4)-
beta-glucanase: 125 U/g⁽³⁾

Alpha-amylase: 1000 U/g⁽⁴⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-xylanase: 3975 U/g⁽²⁾endo-1,3(4)-beta-
glucanase: 125 U/g⁽³⁾alpha-amylase: 1000 U/g⁽⁴⁾

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides, (mainly arabinoxylans and beta-glucans) e.g. containing more than 30% wheat and 20% barley and 20% rye.

6.1.2004 ^(I)(a)

44

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Alpha-amylase
EC 3.2.1.1.

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 250 U/g⁽⁵⁾

endo-1,4-beta-xylanase: 400 U/g⁽⁶⁾

alpha-amylase: 1000 U/g⁽⁷⁾

Piglets

4 months

Endo-1,3(4)-
beta-glucanase: 250 U/g⁽⁵⁾

Endo-1,4-beta-
xylanase: 400 U/g⁽⁶⁾

Alpha-amylase: 1000 U/g⁽⁷⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 250 U/g⁽⁵⁾endo-1,4-beta-xylanase: 400 U/g⁽⁶⁾alpha-amylase: 1000 U/g⁽⁷⁾.

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 50% barley.

6.1.2004 ^(I)(a)

45

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Alpha-amylase
EC 3.2.1.1.

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD.135) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 250 U/g⁽⁸⁾

endo-1,4-beta-xylanase: 400 U/g⁽⁹⁾

alpha-amylase: 1000 U/g⁽¹⁰⁾

Piglets

4 months

Endo-1,3(4)-
beta-glucanase: 250 U/g⁽⁸⁾

Endo-1,4-beta-
xylanase: 400 U/g⁽⁹⁾

Alpha-amylase: 1000 U/g⁽¹⁰⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 250 U/g⁽⁸⁾endo-1,4-beta-xylanase: 400 U/g⁽⁹⁾alpha-amylase: 1000 U/g⁽¹⁰⁾.

3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 35% barley.

6.1.2004 ^(I)(a)

46

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Polygalacturonase
EC 3.2.1.15

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD.135) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 400 U/g⁽¹¹⁾

endo-1,4-beta-xylanase: 400 U/g⁽¹²⁾

polygalacturonase: 50 U/g⁽¹³⁾

Pigs for fattening

-

Endo-1,3(4)-
beta-glucanase: 400 U/g⁽¹¹⁾

Endo-1,4-beta-
xylanase: 400 U/g⁽¹²⁾

Polygalacturonase: 50 U/g⁽¹³⁾

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 400 U/g⁽¹¹⁾endo-1,4-beta-xylanase: 400 U/g⁽¹²⁾polygalacturonase: 50 U/g⁽¹³⁾.

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% barley.

6.1.2004 ^(I)(a)

47

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Alpha-amylase
EC 3.2.1.1.

Polygalacturonase
EC 3.2.1.15

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD.135), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 150 U/g⁽¹⁴⁾

endo-1,4-beta-xylanase: 4000 U/g⁽¹⁵⁾

alpha-amylase: 1000 U/g⁽¹⁶⁾

polygalacturonase: 25 U/g⁽¹⁷⁾

Piglets

4 months

Endo-1,3(4)-
beta-glucanase: 150 U/g⁽¹⁴⁾

Endo-1,4-beta-
xylanase: 4000 U/g⁽¹⁵⁾

Alpha-amylase: 1000 U/g⁽¹⁶⁾

Polygalacturonase: 25 U/g⁽¹⁷⁾

-

-

-

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 150 U/g⁽¹⁴⁾endo-1,4-beta-xylanase: 4000 U/g⁽¹⁵⁾alpha-amylase: 1000 U/g⁽¹⁶⁾polygalacturonase: 25 U/g⁽¹⁷⁾.

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 20% barley and 35% wheat.

6.1.2004 ^(I)(a)

48

Alpha-amylase
EC 3.2.1.1.

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Preparation of alpha-amylase and endo-1,3(4)-
beta-glucanase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of:

Coated form:
alpha-amylase: 200 KNU⁽¹⁸⁾/g
endo-1,3(4)-beta-
glucanase: 350 FBG⁽¹⁹⁾/g

Liquid form:
alpha-amylase: 130 KNU⁽¹⁸⁾/ml
endo-1,3(4)-beta-
glucanase: 225 FBG⁽¹⁹⁾/ml

Chickens for fattening

-

10 KNU⁽¹⁸⁾

17 FBG⁽¹⁹⁾

40 KNU⁽¹⁸⁾

70 FBG⁽¹⁹⁾

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

20 KNU⁽¹⁸⁾35 FBG⁽¹⁹⁾

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 40% barley.

1.4.2004 ^(I)(b)

Turkeys for fattening

-

40 KNU⁽¹⁸⁾

70 FBG⁽¹⁹⁾

80 KNU⁽¹⁸⁾

140 FBG⁽¹⁹⁾

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

40 KNU⁽¹⁸⁾70 FBG⁽¹⁹⁾

3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 40% barley.

1.4.2004 ^(I)(b)

49

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
xylanase
EC 3.2.1.8.

Alpha-amylase
EC 3.2.1.1.

Bacillolysin
EC 3.4.24.28

Polygalacturonase
EC 3.2.1.15

Preparation of endo-1,
3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-
xylanase produced by Trichoderma longibrachiatum (IMI SD.135), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 150 U/g⁽²⁰⁾

endo-1,4-beta-xylanase: 1500 U/g⁽²¹⁾

alpha-amylase: 500 U/g⁽²²⁾

bacillolysin: 800 U/g⁽²³⁾

polygalacturonase: 50 U/g⁽²⁴⁾

Chickens for fattening

-

endo-1,3(4)-
beta-glucanase: 150 U⁽²⁰⁾

endo-1,4-beta-
xylanase: 1500 U⁽²¹⁾

alpha-amylase: 500 U⁽²²⁾

bacillolysin: 800 U⁽²³⁾

polygalacturonase: 50 U⁽²⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 150 U⁽²⁰⁾endo-1,4-beta-xylanase: 1500 U⁽²¹⁾alpha-amylase: 500 U⁽²²⁾bacillolysin: 800 U⁽²³⁾polygalacturonase: 50 U⁽²⁴⁾.

3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 30% wheat.

17.7.2004 ^(I)(c)

Laying hens

-

endo-1,3(4)-
beta-glucanase: 150 U⁽²⁰⁾

endo-1,4-beta-
xylanase: 1500 U⁽²¹⁾

alpha-amylase: 500 U⁽²²⁾

bacillolysin: 800 U⁽²³⁾

polygalacturonase: 50 U⁽²⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 150 U⁽²⁰⁾endo-1,4-beta-xylanase: 1500 U⁽²¹⁾alpha-amylase: 500 U⁽²²⁾bacillolysin: 800 U⁽²³⁾polygalacturonase: 50 U⁽²⁴⁾.

3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 30% wheat.

17.7.2004 ^(I)(c)

50

6-phytase
EC 3.1.3.26.

Preparation of 6-phytase produced by Aspergillus oryzae (DSL 11857) having a minimum activity of:

Coated form: 2500 FYT⁽¹⁾/g

Liquid form: 5000 FYT⁽¹⁾/g

Chickens for fattening

-

250 FYT⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
500 to 1000 FYT⁽¹⁾

3.For use in compound feed containing more than 0,25% phytin bound phosphorus.

17.7.2004 ^(I)(c)

Laying hens

-

250 FYT⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
500 to 1000 FYT⁽¹⁾

3.For use in compound feed containing more than 0,25% phytin bound phosphorus.

17.7.2004 ^(I)(c)

Turkeys for fattening

-

250 FYT⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
500 to 1000 FYT⁽¹⁾

3.For use in compound feed containing more than 0,25% phytin bound phosphorus.

17.7.2004 ^(I)(c)

Piglets

2 months

500 FYT⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
500 to 1000 FYT⁽¹⁾

3.For use in compound feed containing more than 0,25% phytin bound phosphorus.

17.7.2004 ^(I)(c)

Pigs for fattening

-

500 FYT⁽¹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
500 to 1000 FYT⁽¹⁾

3.For use in compound feed containing more than 0,25% phytin bound phosphorus.

17.7.2004 ^(I)(c)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)

(a) First authorisation Commission Regulation (EC) N° 2690/1999 (OJ L 326, 18.12.1999)
(b) First authorisation Commission Regulation (EC) N° 654/2000 (OJ L 79, 30.3.2000)
(c) First authorisation Commission Regulation (EC) N° 1353/2000 (OJ L 155, 28.6.2000)

(1) 1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5,5 and 37°C.
(2) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(3) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(4) 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37°C.
(5) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(6) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(7) 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37°C.
(8) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(9) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(10) 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37°C.
(11) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(12) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(13) 1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40°C.
(14) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(15) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(16) 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37°C.
(17) 1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40°C.
(18) 1 KNU is the amount of enzyme which liberates 672 micromoles of reducing sugars (glucose equivalent) from soluble starch per minute at pH 5,6 and 37°C.
(19) 1 FBG is the amount of enzyme which liberates one micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.

[back to Directive text]

N°	Additive	Chemical name, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

43	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Alpha-amylase EC 3.2.1.1.	Preparation of endo-1,4- beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135), endo-1,3(4)-beta- glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of: endo-1,4-beta-xylanase: 3975 U/g⁽²⁾ endo-1,3(4)-beta- glucanase: 125 U/g⁽³⁾ alpha-amylase: 1000 U/g⁽⁴⁾	Piglets	4 months	Endo-1,4-beta- xylanase: 3975 U/g⁽²⁾ Endo-1,3(4)- beta-glucanase: 125 U/g⁽³⁾ Alpha-amylase: 1000 U/g⁽⁴⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,4-beta-xylanase: 3975 U/g⁽²⁾endo-1,3(4)-beta- glucanase: 125 U/g⁽³⁾alpha-amylase: 1000 U/g⁽⁴⁾ 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides, (mainly arabinoxylans and beta-glucans) e.g. containing more than 30% wheat and 20% barley and 20% rye.	6.1.2004 ^(I)(a)
44	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Alpha-amylase EC 3.2.1.1.	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of: endo-1,3(4)-beta- glucanase: 250 U/g⁽⁵⁾ endo-1,4-beta-xylanase: 400 U/g⁽⁶⁾ alpha-amylase: 1000 U/g⁽⁷⁾	Piglets	4 months	Endo-1,3(4)- beta-glucanase: 250 U/g⁽⁵⁾ Endo-1,4-beta- xylanase: 400 U/g⁽⁶⁾ Alpha-amylase: 1000 U/g⁽⁷⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 250 U/g⁽⁵⁾endo-1,4-beta-xylanase: 400 U/g⁽⁶⁾alpha-amylase: 1000 U/g⁽⁷⁾. 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 50% barley.	6.1.2004 ^(I)(a)
45	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Alpha-amylase EC 3.2.1.1.	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD.135) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of: endo-1,3(4)-beta- glucanase: 250 U/g⁽⁸⁾ endo-1,4-beta-xylanase: 400 U/g⁽⁹⁾ alpha-amylase: 1000 U/g⁽¹⁰⁾	Piglets	4 months	Endo-1,3(4)- beta-glucanase: 250 U/g⁽⁸⁾ Endo-1,4-beta- xylanase: 400 U/g⁽⁹⁾ Alpha-amylase: 1000 U/g⁽¹⁰⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 250 U/g⁽⁸⁾endo-1,4-beta-xylanase: 400 U/g⁽⁹⁾alpha-amylase: 1000 U/g⁽¹⁰⁾. 3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 35% barley.	6.1.2004 ^(I)(a)
46	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Polygalacturonase EC 3.2.1.15	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD.135) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: endo-1,3(4)-beta- glucanase: 400 U/g⁽¹¹⁾ endo-1,4-beta-xylanase: 400 U/g⁽¹²⁾ polygalacturonase: 50 U/g⁽¹³⁾	Pigs for fattening	-	Endo-1,3(4)- beta-glucanase: 400 U/g⁽¹¹⁾ Endo-1,4-beta- xylanase: 400 U/g⁽¹²⁾ Polygalacturonase: 50 U/g⁽¹³⁾	- - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 400 U/g⁽¹¹⁾endo-1,4-beta-xylanase: 400 U/g⁽¹²⁾polygalacturonase: 50 U/g⁽¹³⁾. 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% barley.	6.1.2004 ^(I)(a)
47	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Alpha-amylase EC 3.2.1.1. Polygalacturonase EC 3.2.1.15	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD.135), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: endo-1,3(4)-beta- glucanase: 150 U/g⁽¹⁴⁾ endo-1,4-beta-xylanase: 4000 U/g⁽¹⁵⁾ alpha-amylase: 1000 U/g⁽¹⁶⁾ polygalacturonase: 25 U/g⁽¹⁷⁾	Piglets	4 months	Endo-1,3(4)- beta-glucanase: 150 U/g⁽¹⁴⁾ Endo-1,4-beta- xylanase: 4000 U/g⁽¹⁵⁾ Alpha-amylase: 1000 U/g⁽¹⁶⁾ Polygalacturonase: 25 U/g⁽¹⁷⁾	- - - -	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 150 U/g⁽¹⁴⁾endo-1,4-beta-xylanase: 4000 U/g⁽¹⁵⁾alpha-amylase: 1000 U/g⁽¹⁶⁾polygalacturonase: 25 U/g⁽¹⁷⁾. 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 20% barley and 35% wheat.	6.1.2004 ^(I)(a)
48	Alpha-amylase EC 3.2.1.1. Endo-1,3(4)-beta- glucanase EC 3.2.1.6.	Preparation of alpha-amylase and endo-1,3(4)- beta-glucanase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of: Coated form: alpha-amylase: 200 KNU⁽¹⁸⁾/g endo-1,3(4)-beta- glucanase: 350 FBG⁽¹⁹⁾/g Liquid form: alpha-amylase: 130 KNU⁽¹⁸⁾/ml endo-1,3(4)-beta- glucanase: 225 FBG⁽¹⁹⁾/ml	Chickens for fattening	-	10 KNU⁽¹⁸⁾ 17 FBG⁽¹⁹⁾	40 KNU⁽¹⁸⁾ 70 FBG⁽¹⁹⁾	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 20 KNU⁽¹⁸⁾35 FBG⁽¹⁹⁾ 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 40% barley.	1.4.2004 ^(I)(b)
48			Turkeys for fattening	-	40 KNU⁽¹⁸⁾ 70 FBG⁽¹⁹⁾	80 KNU⁽¹⁸⁾ 140 FBG⁽¹⁹⁾	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 40 KNU⁽¹⁸⁾70 FBG⁽¹⁹⁾ 3.For use in compound feed containing cereals rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 40% barley.	1.4.2004 ^(I)(b)
49	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- xylanase EC 3.2.1.8. Alpha-amylase EC 3.2.1.1. Bacillolysin EC 3.4.24.28 Polygalacturonase EC 3.2.1.15	Preparation of endo-1, 3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta- xylanase produced by Trichoderma longibrachiatum (IMI SD.135), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: endo-1,3(4)-beta- glucanase: 150 U/g⁽²⁰⁾ endo-1,4-beta-xylanase: 1500 U/g⁽²¹⁾ alpha-amylase: 500 U/g⁽²²⁾ bacillolysin: 800 U/g⁽²³⁾ polygalacturonase: 50 U/g⁽²⁴⁾	Chickens for fattening	-	endo-1,3(4)- beta-glucanase: 150 U⁽²⁰⁾ endo-1,4-beta- xylanase: 1500 U⁽²¹⁾ alpha-amylase: 500 U⁽²²⁾ bacillolysin: 800 U⁽²³⁾ polygalacturonase: 50 U⁽²⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 150 U⁽²⁰⁾endo-1,4-beta-xylanase: 1500 U⁽²¹⁾alpha-amylase: 500 U⁽²²⁾bacillolysin: 800 U⁽²³⁾polygalacturonase: 50 U⁽²⁴⁾. 3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 30% wheat.	17.7.2004 ^(I)(c)
49			Laying hens	-	endo-1,3(4)- beta-glucanase: 150 U⁽²⁰⁾ endo-1,4-beta- xylanase: 1500 U⁽²¹⁾ alpha-amylase: 500 U⁽²²⁾ bacillolysin: 800 U⁽²³⁾ polygalacturonase: 50 U⁽²⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 150 U⁽²⁰⁾endo-1,4-beta-xylanase: 1500 U⁽²¹⁾alpha-amylase: 500 U⁽²²⁾bacillolysin: 800 U⁽²³⁾polygalacturonase: 50 U⁽²⁴⁾. 3.For use in compound feed containing cereals rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans ), e.g. containing more than 30% wheat.	17.7.2004 ^(I)(c)
50	6-phytase EC 3.1.3.26.	Preparation of 6-phytase produced by Aspergillus oryzae (DSL 11857) having a minimum activity of: Coated form: 2500 FYT⁽¹⁾/g Liquid form: 5000 FYT⁽¹⁾/g	Chickens for fattening	-	250 FYT⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 FYT⁽¹⁾ 3.For use in compound feed containing more than 0,25% phytin bound phosphorus.	17.7.2004 ^(I)(c)
			Laying hens	-	250 FYT⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 FYT⁽¹⁾ 3.For use in compound feed containing more than 0,25% phytin bound phosphorus.	17.7.2004 ^(I)(c)
			Turkeys for fattening	-	250 FYT⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 FYT⁽¹⁾ 3.For use in compound feed containing more than 0,25% phytin bound phosphorus.	17.7.2004 ^(I)(c)
			Piglets	2 months	500 FYT⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 FYT⁽¹⁾ 3.For use in compound feed containing more than 0,25% phytin bound phosphorus.	17.7.2004 ^(I)(c)
			Pigs for fattening	-	500 FYT⁽¹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 500 to 1000 FYT⁽¹⁾ 3.For use in compound feed containing more than 0,25% phytin bound phosphorus.	17.7.2004 ^(I)(c)