70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (6)

N°
Additive Chemical name, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

51

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG S-15136) having a minimum activity of:
100 IU/g⁽²⁾

Chickens for fattening

-

10 IU⁽²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

10 IU⁽²⁾

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 40% wheat.

17.7.2004 ^(I)(c)

Piglets

2 months

10 IU⁽²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

10 IU⁽²⁾

3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 40% wheat.

31.5.2005 ^(I)(b)

52

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.8.

Alpha-amylase
EC 3.2.1.1.

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of:

liquid form:

endo-1,3(4)-beta-
glucanase: 10000 U/ml⁽³⁾

endo-1,4-beta-
glucanase: 120000 U/ml⁽⁴⁾

alpha-amylase: 400 U/ml⁽⁵⁾

Chickens for fattening

-

Endo-1,3(4)-
beta-glucanase: 1000 U⁽³⁾

Endo-1,4-
beta-glucanase: 12000 U⁽⁴⁾

Alpha-amylase: 40 U⁽⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-
glucanase: 1000 to 2000 U⁽³⁾endo-1,4-beta-
glucanase: 12000 to 24000 U⁽⁴⁾alpha-amylase: 40 to 80 U⁽⁵⁾.

3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 15% sorghum and 5% maize.

17.7.2004 ^(I)(c)

53

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.4.

Alpha-amylase
EC 3.2.1.1.

Bacillolysine
EC 3.4.24.28.

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-
xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 2350 U/g⁽⁶⁾

endo-1,4-beta-
glucanase: 4000 U/g⁽⁷⁾

alpha-amylase: 400 U/g⁽⁸⁾

bacillolysine: 450 U/g⁽⁹⁾

endo-1,4-beta-
xylanase : 20000 U/g⁽¹⁰⁾

Piglets

2 months

Endo-1,3(4)-beta-
glucanase: 2350 U⁽⁶⁾

Endo-1,4-beta-
glucanase: 4000 U⁽⁷⁾

Alpha-amylase: 400 U⁽⁸⁾

Bacillolysine: 450 U⁽⁹⁾

Endo-1,4-beta-
xylanase : 20000 U⁽¹⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 2350 U⁽⁶⁾endo-1,4-beta-
glucanase: 4000 U⁽⁷⁾alpha-amylase: 400 U⁽⁸⁾bacillolysine: 450 U⁽⁹⁾endo-1,4-beta-
xylanase : 20000 U⁽¹⁰⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 25% barley and 20% maize.

23.11.2004 ^(I)(c)

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 1175 U⁽⁶⁾

Endo-1,4-beta-
glucanase: 2000 U/g⁽⁷⁾

Alpha-amylase: 200 U/g⁽⁸⁾

Bacillolysine: 225 U/g⁽⁹⁾

Endo-1,4-beta-
xylanase : 10000 U/g⁽¹⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 1175-2350 U⁽⁶⁾endo-1,4-beta-
glucanase: 2000-4000 U⁽⁷⁾alpha-amylase: 200-400 U⁽⁸⁾bacillolysine: 225-450 U⁽⁹⁾endo-1,4-beta-
xylanase : 10000-20000 U⁽¹⁰⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 45% wheat.

23.11.2004 ^(I)(c)

54

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.4.

Alpha-amylase
EC 3.2.1.1.

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and endo-1,4-beta-
xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 10000 U/g⁽¹¹⁾

endo-1,4-beta-
glucanase: 120000 U/g⁽¹²⁾

alpha-amylase: 400 U/g⁽¹³⁾

endo-1,4-beta-
xylanase : 210000 U/g⁽¹⁴⁾

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 1000 U⁽¹¹⁾

Endo-1,4-beta-
glucanase: 12000 U/g⁽¹²⁾

Alpha-amylase: 40 U/g⁽¹³⁾

Endo-1,4-beta-
xylanase : 21000 U/g⁽¹⁴⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 1000-2000 U⁽¹¹⁾endo-1,4-beta-
glucanase: 12000-24000 U⁽¹²⁾alpha-amylase: 40-80 U⁽¹³⁾endo-1,4-beta-
xylanase : 21000-42000 U⁽¹⁴⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 45% wheat.

23.11.2004 ^(I)(c)

Turkeys for fattening

-

Endo-1,3(4)-beta-
glucanase: 500 U⁽¹¹⁾

Endo-1,4-beta-
glucanase: 6000 U/g⁽¹²⁾

Alpha-amylase: 20 U/g⁽¹³⁾

Endo-1,4-beta-
xylanase : 10500 U/g⁽¹⁴⁾

-

1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 500 to 1500 U⁽¹¹⁾endo-1,4-beta-
glucanase: 6000 to 18000 U⁽¹²⁾alpha-amylase: 20 to 60 U⁽¹³⁾endo-1,4-beta-
xylanase : 10500 to 31500 U⁽¹⁴⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 30% wheat.

13.10.2005 ^(II)(d)

55

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.4.

Alpha-amylase
EC 3.2.1.1.

Bacillolysine
EC 3.4.24.28.

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 3000 U/g⁽¹⁵⁾

endo-1,4-beta-
glucanase: 5000 U/g⁽¹⁶⁾

alpha-amylase: 540 U/g⁽¹⁷⁾

bacillolysine: 450 U/g⁽¹⁸⁾

Piglets

2 months

Endo-1,3(4)-beta-
glucanase: 1500 U⁽¹⁵⁾

Endo-1,4-beta-
glucanase: 2500 U/g⁽¹⁶⁾

Alpha-amylase: 270 U/g⁽¹⁷⁾

Bacillolysine: 225 U/g⁽¹⁸⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta-
glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾

3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 35% wheat and 15% barley.

23.11.2004 ^(I)(c)

Pigs for fattening

-

Endo-1,3(4)-beta-
glucanase: 1500 U⁽¹⁵⁾

Endo-1,4-beta-
glucanase: 2500 U/g⁽¹⁶⁾

Alpha-amylase: 270 U/g⁽¹⁷⁾

Bacillolysine: 225 U/g⁽¹⁸⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta-
glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾

3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 50% barley.

23.11.2004 ^(I)(c)

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 1500 U⁽¹⁵⁾

Endo-1,4-beta-
glucanase: 2500 U/g⁽¹⁶⁾

Alpha-amylase: 270 U/g⁽¹⁷⁾

Bacillolysine: 225 U/g⁽¹⁸⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta-
glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾

3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 50% maize or 50% wheat.

23.11.2004 ^(I)(c)

laying hens

-

Endo-1,3(4)-beta-
glucanase: 1500 U⁽¹⁵⁾

Endo-1,4-beta-
glucanase: 2500 U/g⁽¹⁶⁾

Alpha-amylase: 270 U/g⁽¹⁷⁾

Bacillolysine: 225 U/g⁽¹⁸⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta-
glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾

3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 40% wheat and 10% rye.

23.11.2004 ^(I)(c)

56

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.4.

Alpha-amylase
EC 3.2.1.1.

Bacillolysine
EC 3.4.24.28.

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 6000 U/g⁽¹⁹⁾

endo-1,4-beta-
glucanase: 3500 U/g⁽²⁰⁾

alpha-amylase: 1400 U/g⁽²¹⁾

bacillolysine: 450 U/g⁽²²⁾

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 6000 U⁽¹⁹⁾

Endo-1,4-beta-
glucanase: 3500 U/g⁽²⁰⁾

Alpha-amylase: 1400 U/g⁽²¹⁾

Bacillolysine: 450 U/g⁽²²⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 6000 U⁽¹⁹⁾endo-1,4-beta-
glucanase: 3500 U⁽²⁰⁾alpha-amylase: 1400 U⁽²¹⁾bacillolysine: 450 U⁽²²⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% barley.

23.11.2004 ^(I)(c)

57

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.4.

Alpha-amylase
EC 3.2.1.1.

Bacillolysine
EC 3.4.24.28.

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 3000 U/g⁽²³⁾

endo-1,4-beta-
glucanase: 9000 U/g⁽²⁴⁾

alpha-amylase: 540 U/g⁽²⁵⁾

bacillolysine: 450 U/g⁽²⁶⁾

Chickens for fattening

-

Endo-1,3(4)-beta-
glucanase: 3000 U⁽²³⁾

Endo-1,4-beta-
glucanase: 9000 U/g⁽²⁴⁾

Alpha-amylase: 540 U/g⁽²⁵⁾

Bacillolysine: 450 U/g⁽²⁶⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 3000 U⁽²³⁾endo-1,4-beta-
glucanase: 9000 U⁽²⁴⁾alpha-amylase: 540 U⁽²⁵⁾bacillolysine: 450 U⁽²⁶⁾

3.For use in compound feed rich in starch and non-starch polysaccharides (mainly cellulose and hemicellulose), e.g. containing more than 20% sunflower meal and 10% soya meal.

23.11.2004 ^(I)(c)

58

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Endo-1,4-beta-
glucanase
EC 3.2.1.4.

Alpha-amylase
EC 3.2.1.1.

Bacillolysine
EC 3.4.24.28.

Preparation of endo-1,
3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of:

endo-1,3(4)-beta-
glucanase: 2350 U/g⁽²⁷⁾

endo-1,4-beta-
glucanase: 5000 U/g⁽²⁸⁾

alpha-amylase: 400 U/g⁽²⁹⁾

bacillolysine: 5000 U/g⁽³⁰⁾

Piglets

2 months

Endo-1,3(4)-beta-
glucanase: 2350 U⁽²⁷⁾

Endo-1,4-beta-
glucanase: 5000 U/g⁽²⁸⁾

Alpha-amylase: 400 U/g⁽²⁹⁾

Bacillolysine: 5000 U/g⁽³⁰⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-
glucanase: 2350 U⁽²⁷⁾endo-1,4-beta-
glucanase: 5000 U⁽²⁸⁾alpha-amylase: 400 U⁽²⁹⁾bacillolysine: 5000 U⁽³⁰⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% barley.

23.11.2004 ^(I)(c)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)
(II) Commission Regulation (EC) N° 2013/2001 of 12 October 2001 (OJ L 272, 13.10.2001)

(a) First authorisation Commission Regulation (EC) N° 1353/2000 (OJ L 155, 28.6.2000)
(b) First authorisation Commission Regulation (EC) N° 937/2001 (OJ L130, 12.5.2001)
(c) First authorisation Commission Regulation (EC) N° 2437/2000 (OJ L 280, 4.11.2000)
(d) First authorisation Commission Regulation (EC) N° 2013/2001 (OJ L 272, 13.10.2001)

(2) 1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30°C.
(3) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(4) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 7,5 and 30°C.
(5) 1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7,4 and 30°C.
(6) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(7) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50°C.
(8) 1 U is the amount of enzyme which hydrolises 1 micromoles of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37°C.
(9) 1 U is the amount of enzyme which makes 1 microgram of azo-casein soluble in trichloracetic acid per minute at pH 7,5 and 37°C.
(10) 1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50°C.
(11) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(12) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50°C.
(13) 1 U is the amount of enzyme which hydrolises 1 micromoles of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37°C.
(14) 1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50°C.
(15) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(16) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50°C.
(17) 1 U is the amount of enzyme which hydrolises 1 micromoles of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37°C.
(18) 1 U is the amount of enzyme which makes 1 microgram of azo-casein soluble in trichloracetic acid per minute at pH 7,5 and 37°C.
(19) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(20) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50°C.
(21) 1 U is the amount of enzyme which hydrolises 1 micromoles of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37°C.
(22) 1 U is the amount of enzyme which makes 1 microgram of azo-casein soluble in trichloracetic acid per minute at pH 7,5 and 37°C.
(23) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(24) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50°C.
(25) 1 U is the amount of enzyme which hydrolises 1 micromoles of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37°C.
(26) 1 U is the amount of enzyme which makes 1 microgram of azo-casein soluble in trichloracetic acid per minute at pH 7,5 and 37°C.
(27) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30°C.
(28) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50°C.
(29) 1 U is the amount of enzyme which hydrolises 1 micromoles of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37°C.
(30) 1 U is the amount of enzyme which makes 1 microgram of azo-casein soluble in trichloracetic acid per minute at pH 7,5 and 37°C.

[back to Directive text]

N°	Additive	Chemical name, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

51	Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG S-15136) having a minimum activity of: 100 IU/g⁽²⁾	Chickens for fattening	-	10 IU⁽²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 10 IU⁽²⁾ 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 40% wheat.	17.7.2004 ^(I)(c)
51	Endo-1,4-beta- xylanase EC 3.2.1.8		Piglets	2 months	10 IU⁽²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: 10 IU⁽²⁾ 3.For use in compound feed rich in non-starch polysaccharides, (mainly arabinoxylans) e.g. containing more than 40% wheat.	31.5.2005 ^(I)(b)
52	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.8. Alpha-amylase EC 3.2.1.1.	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) having a minimum activity of: liquid form: endo-1,3(4)-beta- glucanase: 10000 U/ml⁽³⁾ endo-1,4-beta- glucanase: 120000 U/ml⁽⁴⁾ alpha-amylase: 400 U/ml⁽⁵⁾	Chickens for fattening	-	Endo-1,3(4)- beta-glucanase: 1000 U⁽³⁾ Endo-1,4- beta-glucanase: 12000 U⁽⁴⁾ Alpha-amylase: 40 U⁽⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1000 to 2000 U⁽³⁾endo-1,4-beta- glucanase: 12000 to 24000 U⁽⁴⁾alpha-amylase: 40 to 80 U⁽⁵⁾. 3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20% wheat and 15% sorghum and 5% maize.	17.7.2004 ^(I)(c)
53	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.4. Alpha-amylase EC 3.2.1.1. Bacillolysine EC 3.4.24.28. Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta- xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of: endo-1,3(4)-beta- glucanase: 2350 U/g⁽⁶⁾ endo-1,4-beta- glucanase: 4000 U/g⁽⁷⁾ alpha-amylase: 400 U/g⁽⁸⁾ bacillolysine: 450 U/g⁽⁹⁾ endo-1,4-beta- xylanase : 20000 U/g⁽¹⁰⁾	Piglets	2 months	Endo-1,3(4)-beta- glucanase: 2350 U⁽⁶⁾ Endo-1,4-beta- glucanase: 4000 U⁽⁷⁾ Alpha-amylase: 400 U⁽⁸⁾ Bacillolysine: 450 U⁽⁹⁾ Endo-1,4-beta- xylanase : 20000 U⁽¹⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 2350 U⁽⁶⁾endo-1,4-beta- glucanase: 4000 U⁽⁷⁾alpha-amylase: 400 U⁽⁸⁾bacillolysine: 450 U⁽⁹⁾endo-1,4-beta- xylanase : 20000 U⁽¹⁰⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 25% barley and 20% maize.	23.11.2004 ^(I)(c)
53			Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 1175 U⁽⁶⁾ Endo-1,4-beta- glucanase: 2000 U/g⁽⁷⁾ Alpha-amylase: 200 U/g⁽⁸⁾ Bacillolysine: 225 U/g⁽⁹⁾ Endo-1,4-beta- xylanase : 10000 U/g⁽¹⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1175-2350 U⁽⁶⁾endo-1,4-beta- glucanase: 2000-4000 U⁽⁷⁾alpha-amylase: 200-400 U⁽⁸⁾bacillolysine: 225-450 U⁽⁹⁾endo-1,4-beta- xylanase : 10000-20000 U⁽¹⁰⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 45% wheat.	23.11.2004 ^(I)(c)
54	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.4. Alpha-amylase EC 3.2.1.1. Endo-1,4-beta- xylanase EC 3.2.1.8	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and endo-1,4-beta- xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of: endo-1,3(4)-beta- glucanase: 10000 U/g⁽¹¹⁾ endo-1,4-beta- glucanase: 120000 U/g⁽¹²⁾ alpha-amylase: 400 U/g⁽¹³⁾ endo-1,4-beta- xylanase : 210000 U/g⁽¹⁴⁾	Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 1000 U⁽¹¹⁾ Endo-1,4-beta- glucanase: 12000 U/g⁽¹²⁾ Alpha-amylase: 40 U/g⁽¹³⁾ Endo-1,4-beta- xylanase : 21000 U/g⁽¹⁴⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1000-2000 U⁽¹¹⁾endo-1,4-beta- glucanase: 12000-24000 U⁽¹²⁾alpha-amylase: 40-80 U⁽¹³⁾endo-1,4-beta- xylanase : 21000-42000 U⁽¹⁴⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 45% wheat.	23.11.2004 ^(I)(c)
54			Turkeys for fattening	-	Endo-1,3(4)-beta- glucanase: 500 U⁽¹¹⁾ Endo-1,4-beta- glucanase: 6000 U/g⁽¹²⁾ Alpha-amylase: 20 U/g⁽¹³⁾ Endo-1,4-beta- xylanase : 10500 U/g⁽¹⁴⁾	-	1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 500 to 1500 U⁽¹¹⁾endo-1,4-beta- glucanase: 6000 to 18000 U⁽¹²⁾alpha-amylase: 20 to 60 U⁽¹³⁾endo-1,4-beta- xylanase : 10500 to 31500 U⁽¹⁴⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 30% wheat.	13.10.2005 ^(II)(d)
55	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.4. Alpha-amylase EC 3.2.1.1. Bacillolysine EC 3.4.24.28.	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of: endo-1,3(4)-beta- glucanase: 3000 U/g⁽¹⁵⁾ endo-1,4-beta- glucanase: 5000 U/g⁽¹⁶⁾ alpha-amylase: 540 U/g⁽¹⁷⁾ bacillolysine: 450 U/g⁽¹⁸⁾	Piglets	2 months	Endo-1,3(4)-beta- glucanase: 1500 U⁽¹⁵⁾ Endo-1,4-beta- glucanase: 2500 U/g⁽¹⁶⁾ Alpha-amylase: 270 U/g⁽¹⁷⁾ Bacillolysine: 225 U/g⁽¹⁸⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta- glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾ 3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 35% wheat and 15% barley.	23.11.2004 ^(I)(c)
			Pigs for fattening	-	Endo-1,3(4)-beta- glucanase: 1500 U⁽¹⁵⁾ Endo-1,4-beta- glucanase: 2500 U/g⁽¹⁶⁾ Alpha-amylase: 270 U/g⁽¹⁷⁾ Bacillolysine: 225 U/g⁽¹⁸⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta- glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾ 3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 50% barley.	23.11.2004 ^(I)(c)
			Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 1500 U⁽¹⁵⁾ Endo-1,4-beta- glucanase: 2500 U/g⁽¹⁶⁾ Alpha-amylase: 270 U/g⁽¹⁷⁾ Bacillolysine: 225 U/g⁽¹⁸⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta- glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾ 3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 50% maize or 50% wheat.	23.11.2004 ^(I)(c)
			laying hens	-	Endo-1,3(4)-beta- glucanase: 1500 U⁽¹⁵⁾ Endo-1,4-beta- glucanase: 2500 U/g⁽¹⁶⁾ Alpha-amylase: 270 U/g⁽¹⁷⁾ Bacillolysine: 225 U/g⁽¹⁸⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 1500-3000 U⁽¹⁵⁾endo-1,4-beta- glucanase: 2500-5000 U⁽¹⁷⁾alpha-amylase: 270-540 U⁽¹⁷⁾bacillolysine: 225-450 U⁽¹⁸⁾ 3.For use in compound feed rich in starch and non-starch polysaccharides, e.g. containing more than 40% wheat and 10% rye.	23.11.2004 ^(I)(c)
56	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.4. Alpha-amylase EC 3.2.1.1. Bacillolysine EC 3.4.24.28.	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of: endo-1,3(4)-beta- glucanase: 6000 U/g⁽¹⁹⁾ endo-1,4-beta- glucanase: 3500 U/g⁽²⁰⁾ alpha-amylase: 1400 U/g⁽²¹⁾ bacillolysine: 450 U/g⁽²²⁾	Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 6000 U⁽¹⁹⁾ Endo-1,4-beta- glucanase: 3500 U/g⁽²⁰⁾ Alpha-amylase: 1400 U/g⁽²¹⁾ Bacillolysine: 450 U/g⁽²²⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 6000 U⁽¹⁹⁾endo-1,4-beta- glucanase: 3500 U⁽²⁰⁾alpha-amylase: 1400 U⁽²¹⁾bacillolysine: 450 U⁽²²⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% barley.	23.11.2004 ^(I)(c)
57	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.4. Alpha-amylase EC 3.2.1.1. Bacillolysine EC 3.4.24.28.	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of: endo-1,3(4)-beta- glucanase: 3000 U/g⁽²³⁾ endo-1,4-beta- glucanase: 9000 U/g⁽²⁴⁾ alpha-amylase: 540 U/g⁽²⁵⁾ bacillolysine: 450 U/g⁽²⁶⁾	Chickens for fattening	-	Endo-1,3(4)-beta- glucanase: 3000 U⁽²³⁾ Endo-1,4-beta- glucanase: 9000 U/g⁽²⁴⁾ Alpha-amylase: 540 U/g⁽²⁵⁾ Bacillolysine: 450 U/g⁽²⁶⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 3000 U⁽²³⁾endo-1,4-beta- glucanase: 9000 U⁽²⁴⁾alpha-amylase: 540 U⁽²⁵⁾bacillolysine: 450 U⁽²⁶⁾ 3.For use in compound feed rich in starch and non-starch polysaccharides (mainly cellulose and hemicellulose), e.g. containing more than 20% sunflower meal and 10% soya meal.	23.11.2004 ^(I)(c)
58	Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Endo-1,4-beta- glucanase EC 3.2.1.4. Alpha-amylase EC 3.2.1.1. Bacillolysine EC 3.4.24.28.	Preparation of endo-1, 3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) having a minimum activity of: endo-1,3(4)-beta- glucanase: 2350 U/g⁽²⁷⁾ endo-1,4-beta- glucanase: 5000 U/g⁽²⁸⁾ alpha-amylase: 400 U/g⁽²⁹⁾ bacillolysine: 5000 U/g⁽³⁰⁾	Piglets	2 months	Endo-1,3(4)-beta- glucanase: 2350 U⁽²⁷⁾ Endo-1,4-beta- glucanase: 5000 U/g⁽²⁸⁾ Alpha-amylase: 400 U/g⁽²⁹⁾ Bacillolysine: 5000 U/g⁽³⁰⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta- glucanase: 2350 U⁽²⁷⁾endo-1,4-beta- glucanase: 5000 U⁽²⁸⁾alpha-amylase: 400 U⁽²⁹⁾bacillolysine: 5000 U⁽³⁰⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30% barley.	23.11.2004 ^(I)(c)