70/524/EEC annex 1

COUNCIL DIRECTIVE (70/524/EEC)

of 23 November 1970

concerning additives in feeding-stuffs

(70/524/EEC)

List of other additives authorised on a provisional basis for no longer than four years or five years

in the case of additives which have been the subject of provisional authorisation before 1 April 1998

Enzymes (7)

N°
Additive Chemical name, description Species or category of animal Maximum age Minimum content
Units of activity per kg of complete feedingstuff Maximum content
Units of activity per kg of complete feedingstuff Other previsions Period of authorization

59

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Subtilisin
EC 3.4.21.62

Alpha-amylase
EC 3.2.1.1.

Polygalacturonase
EC 3.2.1.15

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:

endo-1,4-beta-xylanase:
300 U⁽¹⁾/g
endo-1,3(4)-beta-
glucanase:
150 U⁽²⁾/g
subtilisin:
4000 U^(3)/g
alpha-amylase:
400 U⁽⁴⁾/g
polygalacturonase:
25 U⁽⁵⁾/g

Chickens for fattening

-

Endo-1,4-beta-
xylanase:
300 U⁽¹⁾
Endo-1,3(4)-beta-
glucanase:
150 U⁽²⁾
Subtilisin:
4000 U⁽³⁾
Alpha-amylase:
400 U⁽⁴⁾
Polygalacturonase:
25 U⁽⁵⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

Endo-1,4-beta-
xylanase:
300 U⁽¹⁾/g
Endo-1,3(4)-beta-
glucanase:
150 U⁽²⁾/g
Subtilisin:
4000 U^(3)/g
Alpha-amylase:
400 U⁽⁴⁾/g
Polygalacturonase:
25 U⁽⁵⁾/g

3.For use in compound feed rich in starch and non-starch polysaccharides, (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% maize

28.2.2005 ^(I)(a)

60

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Preparation of endo-1,
4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of:

endo-1,4-beta-xylanase:
5000 U⁽⁶⁾/g
endo-1,3(4)-beta-
glucanase:
500 U⁽⁷⁾/g

Chickens for fattening

-

Endo-1,4-beta-
xylanase:
500 U⁽⁶⁾

Endo-1,3(4)-beta-
glucanase:
50 U⁽⁷⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-
xylanase:
500 to 2500 U⁽⁶⁾endo-1,3(4)-beta-
glucanase:
5 to 25 U⁽⁷⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20% barley and 40% wheat

28.2.2005 ^(I)(a)

61

Endo-1,4-beta-
xylanase
EC 3.2.1.8

Endo-1,3(4)-beta-
glucanase
EC 3.2.1.6.

Preparation of endo-1,
4-beta-xylanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:

powder form:
endo-1,4-beta-xylanase:
17000 BXU⁽⁸⁾/g
endo-1,3(4)-beta-
glucanase:
11000 BU⁽⁹⁾/g

Liquid form:
endo-1,4-beta-xylanase:
22000 BXU⁽⁸⁾/g
endo-1,3(4)-beta-
glucanase:
15000 BU⁽⁹⁾/g

Chickens for fattening

-

Endo-1,4-beta-
xylanase:
17000 BXU⁽⁸⁾

Endo-1,3(4)-beta-
glucanase:
11000 BU⁽⁹⁾

-

1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.

2.Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-
xylanase:
17000 BXU⁽⁸⁾
endo-1,3(4)-beta-
glucanase:
11000 BU⁽⁹⁾

3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 40% barley and 55% wheat.

28.2.2005 ^(I)(a)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)

(a) First authorisation Commission Regulation (EC) N° 418/2001 (OJ L 62, 2.3.2001)

(1) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(2) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(3) 1 U is the amount of enzyme which liberates1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40°C.
(4) 1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer per minute at pH 6,5 and 37°C.
(5) 1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from poly D-galacturonic substrate per minute at pH 5,0 and 40°C.
(6) 1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50°C.
(7) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30°C.
(8) 1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50°C.
(9) 1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley glucan per minute at pH 4,8 and 50°C..

[back to Directive text]

N°	Additive	Chemical name, description	Species or category of animal	Maximum age	Minimum content Units of activity per kg of complete feedingstuff	Maximum content Units of activity per kg of complete feedingstuff	Other previsions	Period of authorization

59	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,3(4)-beta- glucanase EC 3.2.1.6. Subtilisin EC 3.4.21.62 Alpha-amylase EC 3.2.1.1. Polygalacturonase EC 3.2.1.15	Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of: endo-1,4-beta-xylanase: 300 U⁽¹⁾/g endo-1,3(4)-beta- glucanase: 150 U⁽²⁾/g subtilisin: 4000 U^(3)/g alpha-amylase: 400 U⁽⁴⁾/g polygalacturonase: 25 U⁽⁵⁾/g	Chickens for fattening	-	Endo-1,4-beta- xylanase: 300 U⁽¹⁾ Endo-1,3(4)-beta- glucanase: 150 U⁽²⁾ Subtilisin: 4000 U⁽³⁾ Alpha-amylase: 400 U⁽⁴⁾ Polygalacturonase: 25 U⁽⁵⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: Endo-1,4-beta- xylanase: 300 U⁽¹⁾/g Endo-1,3(4)-beta- glucanase: 150 U⁽²⁾/g Subtilisin: 4000 U^(3)/g Alpha-amylase: 400 U⁽⁴⁾/g Polygalacturonase: 25 U⁽⁵⁾/g 3.For use in compound feed rich in starch and non-starch polysaccharides, (mainly arabinoxylans and beta-glucans), e.g. containing more than 40% maize	28.2.2005 ^(I)(a)
60	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,3(4)-beta- glucanase EC 3.2.1.6.	Preparation of endo-1, 4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of: endo-1,4-beta-xylanase: 5000 U⁽⁶⁾/g endo-1,3(4)-beta- glucanase: 500 U⁽⁷⁾/g	Chickens for fattening	-	Endo-1,4-beta- xylanase: 500 U⁽⁶⁾ Endo-1,3(4)-beta- glucanase: 50 U⁽⁷⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,4-beta- xylanase: 500 to 2500 U⁽⁶⁾endo-1,3(4)-beta- glucanase: 5 to 25 U⁽⁷⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20% barley and 40% wheat	28.2.2005 ^(I)(a)
61	Endo-1,4-beta- xylanase EC 3.2.1.8 Endo-1,3(4)-beta- glucanase EC 3.2.1.6.	Preparation of endo-1, 4-beta-xylanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of: powder form: endo-1,4-beta-xylanase: 17000 BXU⁽⁸⁾/g endo-1,3(4)-beta- glucanase: 11000 BU⁽⁹⁾/g Liquid form: endo-1,4-beta-xylanase: 22000 BXU⁽⁸⁾/g endo-1,3(4)-beta- glucanase: 15000 BU⁽⁹⁾/g	Chickens for fattening	-	Endo-1,4-beta- xylanase: 17000 BXU⁽⁸⁾ Endo-1,3(4)-beta- glucanase: 11000 BU⁽⁹⁾	-	1.In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. 2.Recommended dose per kg of complete feedingstuff: endo-1,4-beta- xylanase: 17000 BXU⁽⁸⁾ endo-1,3(4)-beta- glucanase: 11000 BU⁽⁹⁾ 3.For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans ), e.g. containing more than 40% barley and 55% wheat.	28.2.2005 ^(I)(a)