STANDARDIZATION OF HORMONE AND VETERINARY DRUG RESIDUE ANALYSIS IN ANIMAL PRODUCTS


Scientific Support Plan for a Sustainable Development Policy.
Standards for Foodproducts.

Monitoring programs: data obtained from the Annual Report of the Institute of Veterina Inspection (Belgium) for 1995.

 

Monitoring Programs 1995

 

1. Hormonal residues

 

1.1. Random research* at slaughterhouses:

Species

Samples

Sample type

Amount

Positives

% positive

Cattle

1 864

Urine-TLC

Fat

Faeces-TLC

Injection site

Milk

Meat

Bile

115

560

1 042

51

54

25

17

6

3

21

3

1

0

0

Total

 

1.8

Calf

63

 

 

 

200

Urine-TLC

Fat

Faeces-TLC

 

plasma-IA-E2

plasma-IA-T

2

1

60

 

200

200

0

0

0

 

44

3

 

 

 

 

22

1.5

Pigs

0

/

/

/

/

Sheep

0

/

/

/

/

Horses

0

/

/

/

/

* in the random research carcasses are chosen at random and not confiscated

 

Hormonal residues: products found

Product

Cattle

Calf

Pigs

Nortestosterone

2

/

/

Methyltestosterone

6

/

/

Chlortestosterone acetate

5

/

/

Chlorandrostenedione

3

/

/

Norethandrolone

8

/

/

Norgestrel

6

/

/

Stanozolol

1

/

/

Fluoxymesterone

4

/

/

Progesterone

2

/

/

Oestradiol

/

44

/

Testosterone

1

3

/

Ethinyloestradiol

2

/

/

 

1.2. Directed control* at slaughterhouses:

Species

Samples

Sample type

Amount

Positives

% Positive

Cattle

5 196

Urine-TLC

Fat

Injection site

Faeces

1 629

2 832

625

110

10

12

137

2

0.6

0.4

22

1.9

Calf

134

Urine-IA

Urine-TLC

Fat

Liver

Faeces

18

36

52

20

8

0

0

0

0

0

0

0

0

0

0

* In the directed control suspected carcasses are analysed and confiscated awaiting the result of the analysis

 

Hormonal residues: products found

Product

Cattle

Nortestosterone

7

Testosterone

104

Methyltestosterone

10

Chlortestosterone acetate

28

Chlorandrostenedione

4

Ethylestrenol

2

Acetoxyprogesterone

3

Norgestrel

10

Norethandrolone

42

Methylboldenone

14

Stanozolol

72

Trenbolone

7

Trenbolone acetate

8

Fluoxymesterone

3

Oestradiol

76

Oestradiol benzoate

3

Progesterone

101

Medroxyprogesterone acetate

3

Caproxyprogesterone

1

Chlormadinone acetate

1

Delmadinone acetate

1

 

2. ß-Agonists or Corticosteroids residues

 

2.1. Random research at slaughterhouses:

Species

Samples

Sample type

Amount

Positives

% Positive

Cattle

1 090

Urine-RIA

Faeces-IA

Liver-IA

Eye

86

30

942

32

2

5

47

0

 

Total 5

Calf

325

Faeces-IA

Liver-IA

4

321

1

5

Total 1.8

Pigs

797

Urine-IA

Urine-TLC

Liver-IA

18

2

777

5

2

10

 

Total 2.1

Sheep

0

/

/

/

/

Horses

0

/

/

/

/

ß-Agonists / Corticosteroids: products found

Product

Cattle

Calf

Pigs

Clenbuterol

7

0

7

Dexamethasone

48

6

14

Betamethasone

1

0

0

 

3. Anti-Hormonal residues

 

3.1. Random research at slaughterhouses:

Species

Samples

Positives

Cattle

288

0

Calf

0

0

Pigs

178

0

Sheep

0

0

Horses

0

0

3.2. Directed control at slaughterhouses:

Species

Samples

Positives

Cattle

0

0

 

4. Non-Hormonal residues

 

4.1. Random research at slaugtherhouses:

 

4.1.1. Antibiotics:

Species

Sample type

Amount

Positives

% Positive

Cattle

Kidney

834

17

2

Calf

Kidney

600

10

1.7

Pigs

Kidney

10 619

220

2.1

Sheep

Kidney

79

0

0

Horses

Kidney

12

0

0

TOTAL

Kidney

12 144

247

2

4.1.2. Sulfonamides:

 

9 samples were positive on a total of 288 analysed samples. These 9 samples were from pigs.

 

4.1.3. Chloramphenicol:

 

Despite the use of chloramphenicol is forbidden, 11 samples on a total of 279 were positive. 4 samples were from calves and 7 from pigs.

 

4.1.4. Oxfendazole-Fenbendazole:

 

On a total of 99 analysed samples, none were positive.

 

4.1.5. Ivermectine:

 

No samples were positive on a total of 100 analysed samples.

 

4.1.6. Tranquillizers:

 

17 samples were positive on a total of 288 analysed samples. These 17 samples were all from pigs.

 

4.1.7. ß-Agonists:

Product

Samples

Positives

Animal species

Clenbuterol

290

0

/

Mabuterol

290

0

/

Salbutamol

290

0

/

Clenproperol

290

0

/

Cimaterol

290

2

1 pig and 1 cattle

 

4.2. Directed control: Non-hormonal residues - antibiotics

Species

Sample type

Amount

Positives

% Positive

Cattle

Kidney

8.621

715

8.3

Calf

Kidney

1.493

225

15.1

Pigs

Kidney

19,551

573

2.9

Sheep

Kidney

6

0

0

Horses

Kidney

279

29

10.4

TOTAL

Kidney

29 950

1 542

5.1

 

5. Random research of poultry:

 

102 samples were analysed for the prescence of

a) antibiotics:
- sulfonamides

- tetracyclines

- enrofloxacine

b) flubendazole

c) -agonists

 

3 samples were positive for the presence of tetracyclines

 

6. Livestock breeding companies:

 

6.1. Directed control for substances with hormonal, -adrenergic or production stimulating action

Species

Companies

Positive companies

Samples

Positive samples

% positive samples

animals destroyed

cattle

48

13

1 658

208*

12.5%

196

calf

6

3

1 090

24

2.2%

0

* 196 samples were positive for forbidden compounds with oestrogenic, androgenic and gestagenic action, 12 samples were positive for compounds with -adrenergic and/or corticosteroid action

 

products found:

Product

Cattle

Fluoxymesterone

14

Norethandrolone

19

Norgestrel

82

Methyltestosteron

9

Nortestosteron

2

Trenbolone

79

Ethinyloestradiol

4

Zeranol

4

Chlorandrostenedione

4

Clenbuterol

21

Dexamethasone

11

Beclomethasone

2

Betamethasone

5

 


[Monitoring Programs]