COUNCIL DIRECTIVE (70/524/EEC)

concerning additives in feeding-stuffs

Micro-organisms (1)

N°	Additive	Chemical formula, description	Species or category of animal	Maximum age	Minimum content CFU/kg of complete feedingstuff	Maximum content CFU/kg of complete feedingstuff	Other previsions	Period of authorization
[E 1700	Bacillus licheniformis (DSM 5749) Bacillus subtilis (DSM 5750) (In a 1/1 ratio)	Mixture of Bacillus licheniformis andBacillus subtilis containing a minimum of 3,2 * 109 CFU/g of the additive (1,6 * 109 CFU/g of each bacterium)	Piglets	2 months	1,28 * 109	3,2 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting.	Without a time limit]1
[E 1701	Bacillus cereus var. toyoi (NCIMB 40112/CNCM I-1012	Preparation of Bacillus cereus var. toyoi containing a minimum of 1 * 1010 CFU/g additive	Piglets	2 months	1 * 109	1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting.	Without a time limit]2
			Sows	From 1 week prior to farrowing until weaning	0,5 * 109	2 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting.	Without a time limit]2

1 Commission Regulation (EC) N° 2437/2000 of 3 November 2000 (OJ L 280, 4.11.2000)
2 Commission Regulation (EC) N° 256/2002 of 12 February 2002 (OJ L 41, 13.2.2002)

N°	Additive	Chemical formula, description	Species or category of animal	Maximum age	Minimum content CFU/kg of complete feedingstuff	Maximum content CFU/kg of complete feedingstuff	Other previsions	Period of authorization
1.	Bacillus cereus var. toyoi NCIMB 40112 CNCM I-1012	Preparation of Bacillus cereus var. toyoi containing a minimum of: 1 * 1010 CFU/g additive	Chickens for fattening	-	0,2 * 109	1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage life and stability to pelleting. May be used in compound feed containing the permitted coccidiostats: monensin sodium, lasolacid sodium, salinomycin sodium, decoquinate, robenidine, narasin, halofuginone.	7.10.2004 (II)(a)
			Laying hens	-	0,2 * 109	1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage life and stability to pelleting.	7.10.2004 (II)(a)
			Calves	Six months	0,5 * 109	1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage life and stability to pelleting.	7.10.2004 (II)(a)
			Cattle for fattening	-	0,2 * 109	0,2 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting. The quantity of Bacillus cereus var. toyoi in the daily ration must not exceed 1,0 * 109 CFU for 100 kg, body weight. Add 0,2 * 109 CFU for each additional 100 kg body weight.	7.10.2004 (II)(a)
			Breeding does	-	0,1 * 109	5 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting. May be used in compound feed containing the permitted coccidiostats: robenidine.	7.10.2004 (II)(a)
			Rabbits for fattening	-	0,1 * 109	5 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting. May be used in compound feed containing the permitted coccidiostats: robenidine, salinomycin sodium.	7.10.2004 (II)(a)
3	Saccharomyces cerevisiae NCYC Sc 47	Preparation of Saccharomyces cerevisiae containing a minimum of: 5 * 109 CFU/g additive	Dairy cows	-	4 * 108	2 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting. The quantity of Saccharomyces cerevisiae in the daily ration must not exceed 5.6 * 109 CFU for 100 kg of bodyweight. Add 8,75 * 109 CFU per each additional 100 kg body weight.	31.5.2005 (I)(b)
			Rabbits for fattening	-	2,5 * 109	5 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting. May be used in compound feed containing the permitted coccidiostats: meticlorpindol.	30.6.2004 (I)(c)
			Sows	-	5 * 109	2,5 * 1010	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting.	30.6.2004 (I)(c)
			Piglets	Four months	5 * 109	1 * 1010	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting.	30.6.2004 (I)(c)
5	Saccharomyces cerevisiae CBS 493.94	Preparation of Saccharomyces cerevisiae containing a minimum of: 1 * 108 CFU/g additive	Cattle for fattening	-	1,7 * 108	1,7 * 108	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. The quantity of Saccharomyces cerevisiae in the daily ration must not exceed 7,5 * 108 CFU for 100 kg of body weight. Add 1 * 108 CFU for each extra 100 kg of body weight.	30.6.2004 (I)(d)
			Calves	Six months	2 * 108	2 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.	30.6.2004 (I)(c)
			Dairy cows	-	5 * 107	3,5 * 108	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life and stability to pelleting. The quantity of Saccharomyces cerevisiae in the daily ration must not exceed 5.6 * 109 CFU for 100 kg of bodyweight. Add 8,75 * 109 CFU per each additional 100 kg body weight.	31.5.2005 (I)(b)
6	Saccharomyces cerevisiae CNCM I-1079	Preparation of Saccharomyces cerevisiae containing a minimum of: 2 * 1010 CFU/g additive	Sows	-	2 * 109	1 * 1010	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.	30.6.2004 (I)(c)
			Piglets	Four months	6 * 109	3 * 1010	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.	30.6.2004 (I)(c)
7	Saccharomyces cerevisiae CNCM I-1077	Preparation of Saccharomyces cerevisiae containing a minimum of: 2 * 1010 CFU/g additive	Dairy cows	-	5,5 * 108	2,1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. The quantity of Saccharomyces cerevisiae in the daily ration must not exceed 8,4 * 109 CFU for 100 kg of body weight. Add 1,8 * 109 CFU for each extra 100 kg of body weight.	30.6.2004 (I)(c)
			Cattle for fattening	-	1 * 109	1,5 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. The quantity of Saccharomyces cerevisiae in the daily ration must not exceed 4,6 * 109 CFU for 100 kg of body weight. Add 2 * 109 CFU for each extra 100 kg of body weight.	30.6.2004 (I)(c)
8	Enterococcus faecium ATCC 53519 Enterococcus faecium ATCC 55593 (In a 1/1 ratio)	Mixture of encapsulated Enterococcus faecium (ATCC 53519) and encapsulated Enterococcus faecium (ATCC 55593) containing a minimum of 2 * 108 CFU/g of the additive (i.e. a minimum of 1 * 108 CFU/g of each bacterium)	Chickens for fattening	-	1 * 108	1 * 108	In the directions for use of the additive and pre-mixture, indicate the storage life and stability to pelleting. May be used in compound feed containing the permitted coccidiostats: amprolium, decoquinate, halofuginone, lasalocid sodium, maduramicin ammonium, monensin sodium, narasin, nicarbazin, narasin/nicarbazin, salinomycin sodium.	30.6.2004 (I)(c)
9	Pediococcus acidilactici CNCM MA 18/5M	Preparation of Pediococcus acidilactici containing a minimum of 1 * 1010 CFU/g additive	Chickens for fattening	-	1 * 109	1 * 1010	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting. May be used in compound feed containing the permitted coccidiostats: amprolium, meticlorpindol, decoquinate, halofuginone, narasin, salinomycin sodium, nicarbazin, maduramicin ammonium, diclazuril.	30.6.2004 (I)(d)
			Piglets	Four months	1 * 109	1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.	30.6.2004 (I)(d)
			Pigs for fattening	-	1 * 109	1 * 109	In the directions for use of the additive and pre-mixture, indicate the storage temperature, storage life, and stability to pelleting.	30.6.2004 (I)(d)

(I) Commission Regulation (EC) N° 2200/2001 of 17 October 2001 (OJ L 299, 15.11.2001)
(II) Commission Regulation (EC) N° 256/2002 of 12 February 2002 (OJ L 41, 13.2.2002)

(a) First authorisation Commission Regulation (EC) N° 1411/1999 (OJ L 164, 30.6.1999)
(b) First authorisation Commission Regulation (EC) N° 937/2001 (OJ L 130, 12.5.2001)
(c) First authorisation Commission Regulation (EC) N° 1436/98 (OJ L 191, 7.7.1998)
(d) First authorisation Commission Regulation (EC) N° 866/1999 (OJ L 108, 27.4.1999)